Patents by Inventor Charles Johannes
Charles Johannes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230374443Abstract: A Saccharomyces yeast cell, preferably a Saccharomyces cerevisae yeast cell, comprising one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrate reductase activity and/or one or more nucleic acid sequences encoding an enzyme having NADH-dependent nitrite reductase activity. An anaerobic fermentation process for the production of a fermentation product using such Saccharomyces yeast cell and a fermentation co-product produced.Type: ApplicationFiled: October 5, 2021Publication date: November 23, 2023Inventors: Hans Marinus Charles Johannes DE BRUIJN, Phillip E. BUREMAN, Evert Tjeerd VAN RIJ, René Marcel DE JONG
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Patent number: 11667935Abstract: The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: a) Inoculating a optionally diluted carbon source with the cell; b) optionally fermenting the reactor in batch mode; c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor; d) after sufficient fermentation time, isolation of fermentation product from the reactor, e) optionally keeping the remaining fraction after isolation of step d) as spent broth; and f) optionally using the spent broth in step a) to dilute the carbon source.Type: GrantFiled: November 12, 2020Date of Patent: June 6, 2023Assignee: DSM IP ASSETS B.V.Inventors: Hans Marinus Charles Johannes De Bruijn, Wilhelmus Theodorus Antonius Maria De Laat, Paul Klaassen
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Patent number: 11655485Abstract: The invention relates to a process for the production of ethanol comprising fermenting a (optionally liquefied) corn slurry under anaerobic conditions in the presence of a recombinant yeast; and recovering the ethanol, wherein said recombinant yeast functionally expresses a heterologous nucleic acid sequence encoding a glucoamylase having an amino acid sequence according to SEQ ID NO: 1 or which glucoamylase is a functional homologue thereof having a sequence identity of at least 80%, or which glucoamylase is a functional homologue which is derived, by way of one or more amino acid substitutions, deletions or insertions, from the amino acid sequence of SEQ ID NO: 1, and wherein the process comprises dosing a glucoamylase at a concentration of 0.05 g/L or less.Type: GrantFiled: August 15, 2019Date of Patent: May 23, 2023Assignee: DSM IP ASSETS B.V.Inventor: Hans Marinus Charles Johannes De Bruijn
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Patent number: 11624057Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: March 17, 2021Date of Patent: April 11, 2023Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Publication number: 20220380813Abstract: The invention relates to a process for the production of ethanol, the process comprising fermenting of a carbon source composition with a recombinant yeast, wherein the carbon source composition comprises at least glucose and arabinose; and wherein the recombinant yeast comprises arabinose isomerase activity, ribulokinase activity, ribulose phosphate epimerase activity, glycerol uptake activity and glycerol conversion capacity; and wherein the recombinant yeast further comprises a genetic modification leading to the reduction, downregulation, inhibition and/or elimination of the activity of a homologous protein with glycerol-efflux activity; and wherein each of the glucose and the arabinose is converted into ethanol. In addition, the invention relates to a recombinant yeast that can be used in such a process.Type: ApplicationFiled: November 9, 2020Publication date: December 1, 2022Inventors: Ingrid Maria VUGT-VAN LUTZ, Hans Marinus Charles Johannes DE BRUIJN, Rolf POLDERMANS
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Publication number: 20220325253Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 17, 2021Publication date: October 13, 2022Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Patent number: 11414683Abstract: The invention describes a process for the production of ethanol from a composition comprising glucose and between 50 ?M and 100 mM acetic acid, said process comprising fermenting said composition in the presence of a recombinant yeast which is capable to convert acetic acid anaerobically; maintaining the amount of undissociated acetic acid at a value of at least 50 ?M; and recovering the ethanol. Said process is useful for both starch and cellulosic based, acetic acid containing hydrolysates and advantageously results in a greater consumption of acetic acid and thus higher ethanol yields.Type: GrantFiled: September 25, 2018Date of Patent: August 16, 2022Assignee: DSM IP ASSETS B.V.Inventor: Hans Marinus Charles Johannes De Bruijn
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Publication number: 20220204947Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 17, 2021Publication date: June 30, 2022Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Patent number: 11274310Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: September 25, 2018Date of Patent: March 15, 2022Assignee: DSM IP ASSETS B.V.Inventors: Hans Marinus Charles Johannes De Bruijn, Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz
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Patent number: 11203741Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: June 11, 2021Date of Patent: December 21, 2021Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Publication number: 20210317420Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: June 11, 2021Publication date: October 14, 2021Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Publication number: 20210189438Abstract: The invention relates to a process for the production of ethanol comprising fermenting a (optionally liquefied) corn slurry under anaerobic conditions in the presence of a recombinant yeast; and recovering the ethanol, wherein said recombinant yeast functionally expresses a heterologous nucleic acid sequence encoding a glucoamylase having an amino acid sequence according to SEQ ID NO: 1 or which glucoamylase is a functional homologue thereof having a sequence identity of at least 80%, or which glucoamylase is a functional homologue which is derived, by way of one or more amino acid substitutions, deletions or insertions, from the amino acid sequence of SEQ ID NO: 1, and wherein the process comprises dosing a glucoamylase at a concentration of 0.05 g/L or less.Type: ApplicationFiled: August 15, 2019Publication date: June 24, 2021Inventor: Hans Marinus Charles Johannes DE BRUIJN
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Patent number: 10982195Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: GrantFiled: December 18, 2017Date of Patent: April 20, 2021Assignee: DSM IP ASSETS B.V.Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
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Publication number: 20210071205Abstract: The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: a) Inoculating a optionally diluted carbon source with the cell; b) optionally fermenting the reactor in batch mode; c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor; d) after sufficient fermentation time, isolation of fermentation product from the reactor, e) optionally keeping the remaining fraction after isolation of step d) as spent broth; and f) optionally using the spent broth in step a) to dilute the carbon source.Type: ApplicationFiled: November 12, 2020Publication date: March 11, 2021Inventors: Hans Marinus Charles Johannes DE BRUIJN, Wilhelmus Theodorus Antonius Maria DE LAAT, Paul KLAASSEN
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Patent number: 10883122Abstract: The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: a) Inoculating a optionally diluted carbon source with the cell; b) optionally fermenting the reactor in batch mode; c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor; d) after sufficient fermentation time, isolation of fermentation product from the reactor, e) optionally keeping the remaining fraction after isolation of step d) as spent broth; and f) optionally using the spent broth in step a) to dilute the carbon source.Type: GrantFiled: December 17, 2015Date of Patent: January 5, 2021Assignee: DSM IP ASSETS B.V.Inventors: Hans Marinus Charles Johannes De Bruijn, Wilhelmus Theodorus Antonius Maria De Laat, Paul Klaassen
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Publication number: 20200407733Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: September 25, 2018Publication date: December 31, 2020Inventors: Hans Marinus Charles Johannes DE BRUIJN, Paulus Petrus DE WAAL, Ingrid Maria VUGT-VAN LUTZ
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Publication number: 20200283805Abstract: The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less.Type: ApplicationFiled: September 25, 2018Publication date: September 10, 2020Inventors: Paulus Petrus DE WAAL, Ingrid Maria VUGT - VAN LUTZ, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Publication number: 20200270645Abstract: The invention describes a process for the production of ethanol from a composition comprising glucose and between 50 ?M and 100 mM acetic acid, said process comprising fermenting said composition in the presence of a recombinant yeast which is capable to convert acetic acid anaerobically; maintaining the amount of undissociated acetic acid at a value of at least 50 ?M; and recovering the ethanol. Said process is useful for both starch and cellulosic based, acetic acid containing hydrolysates and advantageously results in a greater consumption of acetic acid and thus higher ethanol yields.Type: ApplicationFiled: September 25, 2018Publication date: August 27, 2020Inventor: Hans Marinus Charles Johannes DE BRUIJN
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Patent number: 10631565Abstract: The present invention relates to a rotary cylindrical mould member and a method for manufacturing a rotary cylindrical mould member for moulding food products suitable for consumption. One or more recessed mould cavities (21) are formed in an curved outer surface (22c) of at least one porous mould body (22), defining the shape of the products which are to be produced. An airflow-resistive skin layer is formed by plastic deformation of a cavity surface layer, having a first flow resistance. An inner volume of the porous mould body (22) between an inner surface (22i) and the airflow-resistive skin layer has a porous structure with a second flow resistance to a compressed air flow through the porous inner volume, wherein according to the invention the first flow resistance is essentially the same as the second flow resistance.Type: GrantFiled: August 17, 2017Date of Patent: April 28, 2020Assignee: MAREL FURTHER PROCESSING B.V.Inventors: Johannes Martinus Meulendijks, Dirk Meskendahl, Charles Johannes Maria Geurts, Matheus Martinus Maria Van Der Horst, Martinus Johannes Willebrordus Van Zoelen
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Publication number: 20200024619Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.Type: ApplicationFiled: March 20, 2018Publication date: January 23, 2020Inventors: Paulus Petrus DE WAAL, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN