Abstract: DNA encoding porcine Pro-carboxypeptidase B, vectors comprising the DNA and host cells transformed with the vectors are useful for production of porcine carboxypeptidase B

Abstract: A method for increasing the antibiotic-producing ability of an antibiotic-producing microbial host cell is disclosed. The method involves transforming an antibiotic-producing microorganism with a recombinant DNA cloning vector that codes for the expression of an antibiotic biosynthetic enzyme or other gene product. The gene preferably codes for a product that is rate-limiting in the antibiotic biosynthetic pathway in Streptomyces. Plasmids that are useful for increasing tylosin production in accordance with the present method include plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315. The invention further comprises microorganisms transformed with plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315 and also other microorganisms and vectors used in accordance with the present method.

Abstract: The present invention concerns novel DNA compounds which function as transcriptional activating sequences. Recombinant DNA expression vectors which contain the transcriptional activating sequences and host cells transformed with these expression vectors are also provided. When properly positioned in an expression vector, the novel transcriptional activating sequences enable regulatable expression of an operably linked gene and enhance the structural stability of the expression vector.

Abstract: Plasmid pHKY334, an expression vector for Met-Phe-Pro-Leu-(Asp).sub.4 -Leu-BGH, and host cells containing plasmid pHKY334 are disclosed and claimed.

Abstract: The present invention discloses an .about.1.4 kb Bc1I-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.

Abstract: The present invention discloses selectable, recombinant DNA shuttle vectors for use in streptomycetes and E. coli. The shuttle vectors of the present invention are present at moderately high copy number. The invention further discloses transformants of the aforementioned vectors.

Abstract: The present invention discloses chimeric plasmid SCP2 and SCP2* cloning vectors that are useful in Streptomyces and E. coli. The invention further discloses transformants and a method for detecting transformants of the aforementioned vectors.

Abstract: A novel method for protecting a bacterium from a naturally occurring bacteriophage and the cloning vectors and transformants for carrying out the aforementioned method are disclosed.

Abstract: A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero-specific hosts. The transformants can then be screened for clones containing genes of interest.

Abstract: New glycopeptide antibiotic CUC/CSV which has the formula: ##STR1## wherein R is L-ristosaminyl;R.sub.1 is the disaccharide mannosyl-glucosyl; andR.sub.2 and R.sub.3 are mannosyl, and its salts, particularly the pharmaceutically acceptable salts, are useful new antibiotics are active against gram-positive bacteria and increase feed-efficiency utilization and enhance milk production in ruminants.

Abstract: The present invention is an improvement in the method for stabilizing and selecting host cells containing recombinant DNA. The method involves transforming a host cell with a recombinant DNA cloning vector which contains the .about.2.5 kb BglII cI repressor gene-containing restriction fragment of bacteriophage .lambda. and then lysogenizing the transformed host cell with a lysogenic organism which contains a marker which is lethal or conditionally lethal in the host cell but which is repressed in the transformed host cell by the repressor gene contained in the recombinant DNA cloning vector. The vector additionally contains a gene which codes for the expression of human proinsulin. The invention further comprises related recombinant DNA cloning vectors, transformed host cells and lysogenized transformed host cells.

Abstract: Glycopeptide antibiotic A-4696G is produced by culturing Actinoplanes missouriensis mutant strain ATCC 31681 under submerged aerobic fermentation conditions, and is recovered from the fermentation broth by resin adsorption and chromatographic purification. A-4696G inhibits the growth of bacteria pathogenic to man and animals and also enhances the growth of ruminants by increasing feed utilization.

Abstract: New Actinoplanes missouriensis strains CUC 014 (NRRL 15647) and CSV 558 (NRRL 15646) which operate together to cosynthesize the useful glycopeptide antibiotic CUC/CSV.

Abstract: New glycopeptide antibiotic CUC/CSV which has the formula: ##STR1## wherein R is L-ristosaminyl;R.sub.1 is the disaccharide mannosyl-glucosyl; andR.sub.2 and R.sub.3 are mannosyl, and its salts, particularly the pharmaceutically acceptable salts, are useful new antibiotics are active against gram-positive bacteria and increase feed-efficiency utilization and enhance milk production in ruminants.

Abstract: A novel method for protecting a bacterium from a naturally occurring bacteriophage and the cloning vectors and transformants for carrying out the aforementioned method are disclosed.

Abstract: A method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof. The invention further provides a simple, convenient, and inexpensive method to lyse host cells for purification of intracellular products.

Abstract: Glycopeptide antibiotic A-4696G is produced by culturing Actinoplanes missouriensis mutant strain ATCC 31681 under submerged aerobic fermentation conditions, and is recovered from the fermentation broth by resin adsorption and chromatographic purification. A-4696G inhibits the growth of bacteria pathogenic to man and animals and also enhances the growth of ruminants by increasing feed utilization.

Abstract: An improved method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof.

Abstract: A method of providing maximum frequencies of transduction with Escherichia coli strains, such as E. coli K12 .chi.1776, that are transduced poorly in the exponential growth phase, which comprises transducing the culture in the stationary phase of growth. E. coli K12 .chi.1776 strains modified by transduction using this method are useful as EK2 hosts for biological containment.