Abstract: Provided herein are scaffold biomaterials including a decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure; wherein the decellularized plant or fungal tissue may optionally be at least partially coated or mineralized, wherein the scaffold biomaterial may optionally further include a protein-based hydrogel and/or a polysaccharide-based hydrogel, or both. Also provided herein are methods and uses of such scaffold biomaterials, including methods of manufacture as well as methods and uses for bone tissue engineering, for example.