Abstract: A method and system for distributing access to inventory items available at vendor locations includes user stations transmitting user inquiries on a communications network. Network-connected location servers access a set of inventory availability information associated with inventory items available at their respective location, for example, tables at a restaurant. The host server receives user inquiries regarding availability of items associated with at least some of the location servers and queries a cache server that locally stores a binary subset of the information from the location servers to determine if the requested item is available without having to separately query the database at each location. Particularly, a web server receives and stores information regarding only whether a requested item is available or not available from a particular location rather than how many items are available at the location.

Abstract: Replicons of pestiviruses, in particular replicons of swine fever virus, are engineered to have a defective replication thereby having lost infectivity, and further containing a foreign gene. A replicon contains all the genetic information required for its replication, but lacks essential codons or all codons of at least one of the genes encoding the viral structural proteins E1, E2, Ems or C protein, and consequently cannot generate infectious virus particles. Particular replicons are generated with a mutated gene encoding a modified Npro protein that no longer controls the cell interferon-induction pathway. Another particular replicon lacks the genes encoding all the structural proteins, the p7 protein and the NS2 protein, and has cytopathogenic properties in transduced cells. The replicons provide a new vector system that can be used for vaccination, gene delivery and gene therapy applications in mammals, including humans, as naked RNA or packaged into any form of delivery vehicle.

Abstract: Replicons of pestiviruses, in particular replicons of swine fever virus, are engineered to have a defective replication thereby having lost infectivity, and further containing a foreign gene. A replicon contains all the genetic information required for its replication, but lacks essential codons or all codons of at least one of the genes encoding the viral structural proteins E1, E2, Ems or C protein, and consequently cannot generate infectious virus particles. Particular replicons are generated with a mutated gene encoding a modified Npro protein that no longer controls the cell interferon-induction pathway. Another particular replicon lacks the genes encoding all the structural proteins, the p7 protein and the NS2 protein, and has cytopathogenic properties in transduced cells. The replicons provide a new vector system that can be used for vaccination, gene delivery and gene therapy applications in mammals, including humans, as naked RNA or packaged into any form of delivery vehicle.

Abstract: The present invention concerns replicons of pestiviruses, in particular replicons of swine fever virus, engineered to have a defective replication thereby having lost infectivity, and further containing a foreign gene. A replicon of the invention contains all the genetic information required for its replication, but lacks essential codons or all codons of at least one of the genes encoding the viral structural proteins E1, E2, Ems or C protein, and consequently cannot generate infectious virus particles. Particular replicons are generated with a mutated gene encoding a modified Npro protein that no longer controls the cell interferon-induction pathway. Another particular replicon lacks the genes encoding all the structural proteins, the p7 protein and the NS2 protein, and has cytopathogenic properties in transduced cells.

Abstract: A method and system for distributing access to inventory items available at vendor locations includes user stations transmitting user inquiries on a communications network. Network-connected location servers access a set of inventory availability information associated with inventory items available at their respective location, for example, tables at a restaurant. The host server receives user inquiries regarding availability of items associated with at least some of the location servers and queries a cache server that locally stores a binary subset of the information from the location servers to determine if the requested item is available without having to separately query the database at each location. Particularly, a web server receives and stores information regarding only whether a requested item is available or not available from a particular location rather than how many items are available at the location.

Abstract: A method and system for distributing access to inventory items available at vendor locations includes user stations transmitting user inquiries on a communications network. Network-connected location servers access a set of inventory availability information associated with inventory items available at their respective location, for example, tables at a restaurant. The host server receives user inquiries regarding availability of items associated with at least some of the location servers and queries a cache server that locally stores a binary subset of the information from the location servers to determine if the requested item is available without having to separately query the database at each location. Particularly, a web server receives and stores information regarding only whether a requested item is available or not available from a particular location rather than how many items are available at the location.

Abstract: The present invention relates to a compound comprising a polyelectrolyte and, covalently linked thereto, an immunological adjuvant and/or cell targeting ligand, wherein the covalently linked entity can have both adjuvant and cell targeting characteristics. The compound is used in the preparation of hydrophilic vaccine nanoparticles, which preferably have an antigenic compound or therapeutic agent, or genetic information encoding such compounds or agents entrapped in their matrix, or covalently linked to their surfaces. Vaccine compositions comprising the particles of the invention are advantageous, because a strong and long-lasting immune response is obtained following administration of a single dose. In a preferred embodiment, the polyelectrolyte of the compound is an anionic polymer, and the particle comprises a matrix comprising chitosan.

Abstract: The present invention concerns replicons of pestiviruses, in particular replicons of swine fever virus, engineered to have a defective replication thereby having lost infectivity, and further containing a foreign gene. A replicon of the invention contains all the genetic information required for its replication, but lacks essential codons or all codons of at least one of the genes encoding the viral structural proteins E1, E2, Ems or C protein, and consequently cannot generate infectious virus particles. Particular replicons are generated with a mutated gene encoding a modified Npro protein that no longer controls the cell interferon-induction pathway. Another particular replicon lacks the genes encoding all the structural proteins, the p7 protein and the NS2 protein, and has cytopathogenic properties in transduced cells.

Abstract: Interactive content preservation and customization technology is placed at the broadcast facility to ensure reliable transmission of the interactive content to a local subsystem. An interactive content code detector detects interactive content codes in the video stream at the broadcast facility. The interactive content code detector is placed in the transmission path before the video stream is transmitted to broadcast facility hardware that may strip out, destroy, corrupt or otherwise modify the interactive content and interactive content codes. Once an interactive content code detector detects a code, an interactive broadcast server determines what action to take, and instructs an data insertion unit accordingly. The interactive content codes or interactive content may be placed in a portion of the video that is guaranteed by the broadcast facility to be transmitted, for example, the closed caption region of the vertical blanking interval.

Abstract: An automation server interfaces with broadcast scheduling systems of various types to s automatically synchronize the behavior of interactive applications relative to various broadcast programs, such as television shows and commercials, so as to maintain the appropriate interactive application for whatever broadcast program is airing. This allows for television shows which have an associated interactive application, but which are segmented by commercials (which may have their own interactive applications) to have their interactive application displayed while the television show in on, but not during commercials, while maintaining any state information that has been created during execution of the interactive application. The automation server includes multiple channel interfaces, each having a translator and an event manager. The translator translates native control signals from the scheduling system into a fixed set of atomic commands which represent the lifecycle behavior of a broadcast program.

Abstract: Interactive content preservation and customization technology is placed at the broadcast facility to ensure reliable transmission of the interactive content to a local subsystem. An interactive content code detector detects interactive content codes in the video stream at the broadcast facility. The interactive content code detector is placed in the transmission path before the video stream is transmitted to broadcast facility hardware that may strip out, destroy, corrupt or otherwise modify the interactive content and interactive content codes. Once an interactive content code detector detects a code, an interactive broadcast server determines what action to take, and instructs an data insertion unit accordingly. The interactive content codes or interactive content may be placed in a portion of the video that is guaranteed by the broadcast facility to be transmitted, for example, the closed caption region of the vertical blanking interval.

Abstract: A method and system for distributing access to inventory items available at vendor locations includes user stations transmitting user inquiries on a communications network. Network-connected location servers access a set of inventory availability information associated with inventory items available at their respective location, for example, tables at a restaurant. The host server receives user inquiries regarding availability of items associated with at least some of the location servers and queries a cache server that locally stores a binary subset of the information from the location servers to determine if the requested item is available without having to separately query the database at each location. Particularly, a web server receives and stores information regarding only whether a requested item is available or not available from a particular location rather than how many items are available at the location.