Patents by Inventor Charles P. Lin
Charles P. Lin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120003729Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: ApplicationFiled: July 1, 2011Publication date: January 5, 2012Applicant: VANDERBILT UNIVERSITYInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 8006702Abstract: A method of scanning a laser beam across a set of cells includes during a first interval, scanning a laser beam across a set of cells; and during a second interval, deflecting the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells.Type: GrantFiled: June 2, 2008Date of Patent: August 30, 2011Assignee: The General Hospital CorporationInventor: Charles P Lin
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Patent number: 8003378Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: GrantFiled: March 5, 2009Date of Patent: August 23, 2011Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene LeBoeuf, Chang Y. Chung, David E. Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7947036Abstract: A method of scanning a laser beam across a set of cells includes during a first interval, causing a galvanometric scanner to scan a laser beam across a set of cells; and during a second interval, causing the galvanometric scanner to deflect the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells.Type: GrantFiled: July 23, 2008Date of Patent: May 24, 2011Assignee: The General Hospital CorporationInventor: Charles P Lin
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Publication number: 20110060232Abstract: The present invention provides methods and devices for performing flow cytometry. In one embodiment, blood circulating through one or more retinal blood vessels of a subject is illuminated in-vivo so as to excite a plurality of fluorescent-labeled cells contained in the blood. The fluorescence radiation emitted by the excited cells is then detected and analyzed to count the cells from which fluorescence is detected.Type: ApplicationFiled: May 2, 2008Publication date: March 10, 2011Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Charles P. Lin, Clemens Alt, Israel Veilleux, Daniel Cote
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Publication number: 20110044910Abstract: The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest.Type: ApplicationFiled: March 27, 2009Publication date: February 24, 2011Applicant: THE GENERAL HOSPITAL CORPORATION D/B/A MASSACHUSEETS GENERAL HOSPITALInventors: Charles P. Lin, Alicia L. Carlson, Clemens Alt, David P. Biss, Costas M. Pitsillides, Chunqiang Li
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Publication number: 20100233092Abstract: The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in-vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. In some embodiments, the circulating cells comprise apoptotic cells whose detection can allow, e.g., non-invasive monitoring of the efficacy of a cancer treatment, such as an anti-tumor or an anti-angiogenic therapy.Type: ApplicationFiled: May 24, 2010Publication date: September 16, 2010Applicant: The General Hospital Corporation D/B/A Massachusetts General HospitalInventors: Charles P. Lin, Xunbin Wei, Dorothy Sipkins
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Patent number: 7790443Abstract: A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber.Type: GrantFiled: August 27, 2003Date of Patent: September 7, 2010Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Mark A. Stremler, Alissa Weaver
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Patent number: 7763017Abstract: A method of scanning a laser beam across a set of cells includes during a first interval, scanning a laser beam across a set of cells; and during a second interval, deflecting the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells.Type: GrantFiled: July 26, 2006Date of Patent: July 27, 2010Assignee: The General Hospital CorporationInventor: Charles P. Lin
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Patent number: 7745155Abstract: The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in-vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. In some embodiments, the circulating cells comprise apoptotic cells whose detection can allow, e.g., non-invasive monitoring of the efficacy of a cancer treatment, such as an anti-tumor or an anti-angiogenic therapy.Type: GrantFiled: July 27, 2005Date of Patent: June 29, 2010Assignee: Massachusetts General HospitalInventors: Charles P. Lin, Xunbin Wei, Dorothy Sipkins
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Publication number: 20100049041Abstract: The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest.Type: ApplicationFiled: January 7, 2009Publication date: February 25, 2010Applicant: THE GENERAL HOSPITAL CORPORATION D/B/A MASSACHUSETS GENERAL HOSPITALInventor: Charles P. Lin
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Publication number: 20090215654Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: ApplicationFiled: March 5, 2009Publication date: August 27, 2009Applicant: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7534601Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: GrantFiled: August 27, 2003Date of Patent: May 19, 2009Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Alex Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7491502Abstract: The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest.Type: GrantFiled: December 17, 2004Date of Patent: February 17, 2009Assignee: The General Hospital CorporationInventor: Charles P. Lin
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Publication number: 20080281306Abstract: A method of scanning a laser beam across a set of cells includes during a first interval, causing a galvanometric scanner to scan a laser beam across a set of cells; and during a second interval, causing the galvanometric scanner to deflect the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells.Type: ApplicationFiled: July 23, 2008Publication date: November 13, 2008Applicant: THE GENERAL HOSPITAL CORPORATIONInventor: Charles P. Lin
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Publication number: 20080259422Abstract: A method of scanning a laser beam across a set of cells includes during a first interval, scanning a laser beam across a set of cells; and during a second interval, deflecting the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells.Type: ApplicationFiled: June 2, 2008Publication date: October 23, 2008Applicant: THE GENERAL HOSPITAL CORPORATIONInventor: Charles P. Lin
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Patent number: 7292390Abstract: A confocal microscope system that is inherently fiberoptic compatible is described which has line scanning aided image formation. An incoherent fiberoptic bundle maps a line illumination pattern into a dispersible group of separate sources, and then remaps this confocally selected remitted light to the original line. Fibers, not confocal with the illumination, carry light to be rejected from the image back on itself upon double passing, while separate fibers carry light from non-confocal sample planes. The transformation allows efficient rejection of unwanted photons at a slit aperture. The fiber bundle and an objective lens provide a flexible probe for imaging internal tissue for pathological examination on a cellular level.Type: GrantFiled: April 22, 2004Date of Patent: November 6, 2007Assignee: The General Hospital CorporationInventors: Charles P. Lin, Robert H. Webb
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Patent number: 7264794Abstract: The present invention provides methods and systems for performing in-vivo flow cytometry to obtain desired information regarding one or more cell types of interest flowing through a subject's circulatory system. In one embodiment of the invention, a portion of the subject's circulating blood is illuminated with radiation having multiple wavelength components, and the backscattered radiation generated in response to the excitation radiation is detected at a plurality of scattering angles and analyzed to derive the desired information.Type: GrantFiled: December 17, 2004Date of Patent: September 4, 2007Assignee: The General HospitalInventors: Irene Georgakoudi, Charles P. Lin
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Patent number: 7115120Abstract: Selective photocoagulation of particular cells, tissue or portion of tissue can be aided by monitoring microcavitation within the material exposed to electromagnetic radiation (12). The information gained by the detection of microcavitation events can be used to modulate the intensity of the radiation (12) to prevent significant thermal energy transmission and mechanical damage to cells, tissues or portions of tissues which are not directly exposed, but prone to thermal damage by such energy transmission. Detection of microcavitation also serves as a valuable therapeutic endpoint. The methods of the invention are applicable to laser eye surgery, since the target cells of several therapeutic photocoagulation methods, the retinal pigment epithelial (RPE) cells are proximate to photoreceptor cells. The inventive methods can help prevent the formation of blind spots that can be associated with laser eye surgery. Other fields of laser surgery can also be readily adapted to the methods described herein.Type: GrantFiled: June 1, 2001Date of Patent: October 3, 2006Assignee: The General Hospital CorporationInventor: Charles P. Lin
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Publication number: 20040264897Abstract: A confocal microscope system that is inherently fiberoptic compatible is described which has line scanning aided image formation. An incoherent fiberoptic bundle maps a line illumination pattern into a dispersible group of separate sources, and then remaps this confocally selected remitted light to the original line. Fibers, not confocal with the illumination, carry light to be rejected from the image back on itself upon double passing, while separate fibers carry light from non-confocal sample planes. The transformation allows efficient rejection of unwanted photons at a slit aperture. The fiber bundle and an objective lens provide a flexible probe for imaging internal tissue for pathological examination on a cellular level.Type: ApplicationFiled: April 22, 2004Publication date: December 30, 2004Inventors: Charles P. Lin, Robert H. Webb