Patents by Inventor Charles S. Vann
Charles S. Vann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8889596Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: GrantFiled: August 16, 2013Date of Patent: November 18, 2014Assignee: Life Technologies CorporationInventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
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Publication number: 20140251811Abstract: A device having a first substrate and a second substrate separated from the first substrate to form a volume between the first and second substrates, electrodes disposed on the first and second substrates and facing the volume, each substrate comprising at least one electrode, and a first insulating layer disposed on the first substrate and a second insulating layer disposed on the second substrate to separate the electrodes from the volume.Type: ApplicationFiled: April 28, 2014Publication date: September 11, 2014Applicant: APPLIED BIOSYSTEMS LLCInventors: Charles S. VANN, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Publication number: 20140162264Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: ApplicationFiled: December 2, 2013Publication date: June 12, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
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Publication number: 20140113298Abstract: Apparatuses and methods in which detection is integrated with various liquid processing and environmental control functions to create integrated bioanalysis systems are disclosed. Though the various integrated bioanalysis systems are useful for any number of analysis formats, they are adaptable to high-throughput processing of samples.Type: ApplicationFiled: September 13, 2013Publication date: April 24, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventor: Charles S. Vann
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Publication number: 20140001202Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.Type: ApplicationFiled: May 10, 2013Publication date: January 2, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Publication number: 20130331276Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: ApplicationFiled: August 16, 2013Publication date: December 12, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Mark F. OLDHAM, George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
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Patent number: 8597590Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: GrantFiled: May 27, 2010Date of Patent: December 3, 2013Assignee: Applied Biosystems, LLCInventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
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Patent number: 8568580Abstract: A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber.Type: GrantFiled: July 24, 2008Date of Patent: October 29, 2013Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Maxim G. Brevnov, David W. Ruff, Kenneth J. Livak
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Patent number: 8470149Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.Type: GrantFiled: December 5, 2011Date of Patent: June 25, 2013Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Publication number: 20120138462Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.Type: ApplicationFiled: December 5, 2011Publication date: June 7, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Patent number: 8028591Abstract: Systems and methods are provided that can include a storage and retrieval robot operating under program control to extract a selective number of support beads from a storage or retainment region, for dispensing to titer wells or other vessels for assays and other purposes. According to various embodiments, the storage and retrieval robot can position a capture device over any one or more storage wells containing oligonucleotide or other material support beads, and withdraw or extract those support beads into a collection tube under vacuum pressure or other force. According to various embodiments, a selected number of support beads can be extracted, using a linear motor piston to limit available space for support bead insertion. According to various embodiments, the collected support beads can be dispensed into one or more destination tubes, wells, or other containers, surfaces, vessels, receptacles or mixture containment region, for use in assays or other purposes.Type: GrantFiled: May 11, 2007Date of Patent: October 4, 2011Assignee: Applied Biosystems LLCInventor: Charles S. Vann
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Publication number: 20110177562Abstract: The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge.Type: ApplicationFiled: December 20, 2010Publication date: July 21, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Charles S. Vann, Michael Greenstein, Yuh-Min Chiang
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Publication number: 20110020179Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: ApplicationFiled: May 27, 2010Publication date: January 27, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Min Yue, David M. Liu, Yuh-Min Chiang, Joon-Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Joy Roy, Maryam Shariati, Umberto Ulmanella
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Publication number: 20100300879Abstract: An injection system including a first electrical circuit for concentration of an analyte and a second electrical circuit for injection of the concentrated analyte into an electrophoretic device is described, as well as methods of using the injection system.Type: ApplicationFiled: July 29, 2010Publication date: December 2, 2010Applicant: Applied Biosystems, LLCInventors: Charles S. Vann, Timothy Z. Liu
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Publication number: 20100236930Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.Type: ApplicationFiled: February 19, 2010Publication date: September 23, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
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Publication number: 20100227384Abstract: An apparatus for identifying a target portion of a sample. In some embodiments, the sample can be substantially purified and/or amplified in a test chamber. In some embodiments, the sample can be provided in an amount so that amplification is not necessary. The apparatus generally comprises a test chamber having an input region for receiving a sample and an analysis region. A sample chamber can be defined by at least a portion of the test chamber. A separation mechanism can separate a target portion of the sample from the sample and an analysis chamber can aid in analyzing at least the target portion of the sample.Type: ApplicationFiled: February 1, 2010Publication date: September 9, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Charles S. VANN
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Publication number: 20100167293Abstract: The invention relates to methods and compositions for the detection of targets in a sample.Type: ApplicationFiled: September 17, 2009Publication date: July 1, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Charles S. Vann, Kai Qin Lao, Mark Reed
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Publication number: 20090321259Abstract: The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge.Type: ApplicationFiled: April 30, 2009Publication date: December 31, 2009Applicant: Life Technologies CorporationInventors: Charles S. Vann, Michael Greenstein, yuh-Min Chiang
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Patent number: 7615193Abstract: A bead dispensing system is provided for delivering small amounts of substances onto substrates. The system can include, for example, a movable support structure having an array of spaced-apart projections depending from its lower side. An attraction source, such as a vacuum, magnetic, and/or electrostatic force, is operable at each projection end region to attract and retain one bead. The projection array can be aligned with an array of bead-receiving regions of a substrate, e.g., an array of spaced-apart wells of a micro-plate or card. In one embodiment, a plurality of reagent-carrying beads are picked up, retained at respective projection end regions, and moved to a location over a multi-well plate. The beads are then released in a fashion permitting each bead to land in a respective well. The system of the invention is particularly useful for fabricating arrays of reagents.Type: GrantFiled: November 16, 2004Date of Patent: November 10, 2009Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Dennis Lehto
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Patent number: 7595189Abstract: The device for detecting the binding of two chemical species includes a first plate having a base, multiple optical fibers and a second plate. The base has multiple grooves formed therein. The multiple optical fibers are each disposed within a corresponding one of the multiple grooves. The second plate has multiple channels formed therein. The first plate and the second plate are configured to be placed adjacent to one another such that each the optical fiber is exposed to and traverses the multiple channels.Type: GrantFiled: June 24, 2004Date of Patent: September 29, 2009Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Tim Geiser, Andrew J. Blasband