Abstract: A cost-effective, scalable technique for producing microspheres loaded with biologically active solid proteins is provided. The microspheres degrade over time and release biologically active VEGF, as demonstrated by the proliferation of HUVECs in vitro compared to negative controls. A defined concentration of microspheres can deliver a quantifiable level of VEGF with known release kinetics. The invention can be used with other growth factors and applied to tissue engineering applications such as the regeneration of peripheral nerve, bone, adipose tissue, and solid organs.

Abstract: A cost-effective, scalable technique for producing microspheres loaded with biologically active solid proteins is provided. The microspheres degrade over time and release biologically active VEGF, as demonstrated by the proliferation of HUVECs in vitro compared to negative controls. A defined concentration of microspheres can deliver a quantifiable level of VEGF with known release kinetics. The invention can be used with other growth factors and applied to tissue engineering applications such as the regeneration of peripheral nerve, bone, adipose tissue, and solid organs.

Abstract: The present invention relates to compositions and methods for the stimulation of nerve cell growth and the regeneration of nerve tissue. Using engineered “helper” cells and nerve growth conduits, in vivo stimulation of nerve cell growth, for example, in damaged or diseased tissues, is achieved.

Abstract: An apparatus for loading open top grain hopper rail cars and trucks which eliminates the buildup of a core of small material and grains directly beneath the load out spout. Also, the grain will not build up heat as easily with the core eliminated and a more representative sample, for grading purposes, can be obtained from the hopper car or truck. The spout line buster is a small, stove-shaped chamber which attaches to the end of a grain load out spout and is lowered about six inches into the hopper rail car or truck being loaded. The grain passes through the down spout and enters the spout line buster where the flow of the grain is directed and slowed by a series of baffles or flow guides. In the model designed for loading hopper rail cars, the grain strikes a screen assembly consisting of a cone screen and two diagonal screens.