Abstract: Recombinant beta-casein (&bgr;-CN) proteins and peptides which include the mineral-binding target sequence Xaa-SerP-SerP-Glu-Glu, where SerP is phosphoserine, in which Xaa is any amino acid other than serine or phosphoserine. These phosphopeptides are complexed with minerals, such as calcium, and used as dietary supplements.

Abstract: A human infant formula sufficient to meet the nutritional requirements of a human infant, comprising proteins having substantially the same amino acid sequence and biological properties as human alpha-lactalbumin and human beta-casein. The proteins may be produced from microorganisms, particularly E. coli. A recombinant DNA segment comprising a human milk protein encoding gene; a promoter sequence directing the transcription of the gene, where the promoter sequence is different from the promoter sequence for the gene in the human organism; and a terminator site for the human milk protein encoding gene. A microorganism containing a recombinant DNA segment comprising a human milk protein encoding gene; a promoter sequence directing the transcription of the gene; and a terminator site for the gene.

Abstract: A human infant formula sufficient to meet the nutritional requirements of a human infant, containing proteins having substantially the same amino acid sequence and biological properties as human alpha-lactalbumin and human beta-casein. The proteins may be produced from microorganisms, particularly E. coli. A recombinant DNA segment containing a human milk protein encoding gene; a promoter sequence directing the transcription of the gene, where the promoter sequence is different from the promoter sequence for the gene in the human organism; and a terminator site for the human milk protein encoding gene. A microorganism containing a recombinant DNA segment containing a human milk protein encoding gene; a promoter sequence directing the transcription of the gene; and a terminator site for the gene.