Abstract: h The present disclosure provides compositions, methods, and systems related to template-mediated genome editing and modification. In particular, the present disclosure provides novel genome modification technology involving site-specific chemical modification of a nucleotide to introduce a replication-blocking lesion. The compositions, methods, and systems described herein facilitate efficient site-specific genome modification of a DNA target, while minimizing the unintended edits and cellular toxicity associated with current genome editing approaches.

Abstract: The present disclosure provides compositions, methods, and systems related to genome editing technology. In particular, the present disclosure provides a novel CRISPR-based genome editing technology that involves the generation of abasic sites to facilitate genetic recombination, without the need for breaks in the DNA. The compositions, methods, and systems described herein address many of the drawbacks of currently available approaches, including off-target effects and cellular toxicity.

Abstract: The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.

Abstract: The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.

Abstract: Provided herein are methods and compositions for killing a target bacterium with a CRISPR-Cpf1 system. Also disclosed are engineered bacteriophages.

Abstract: The invention relates to methods of modifying a plastid genome using sequence specific nucleases, as well as reverse transcriptase polypeptides and plastid modification cassettes. The invention further relates to methods of modifying a plastid or a mitochondrial genome using ATP-dependent DNA ligase D (LigD) and DNA-binding protein Ku (Ku). Also included are the plants, plant cells, and seeds produced by these methods.

Abstract: The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.