Abstract: The present invention relates to a system for continuous bioprocessing of adherent cells. The system includes a cell growth chamber; a cell trap in fluid communication with the cell growth chamber, the cell trap including a cell outlet; and a fluid flow system configured to selectively switch between a first mode of operation and a second mode of operation. In the first mode of operation the fluid flow system is configured to direct fluid through the cell growth chamber towards the cell trap such that cells from the cell growth chamber are collected in the cell trap. In the second mode of operation the fluid flow system is configured to direct fluid to the cell trap and through the cell outlet to thereby harvest cells collected in the cell trap.

Abstract: The present invention provides a reversibly cross-linked hydrogel comprising a sample, wherein the sample comprises extracellular nucleic acid. Corresponding methods of preparing, transporting and/or storing the nucleic acid-containing hydrogel, as well as uses thereof, are also provided herein.

Abstract: The present invention relates to the use of a plastically-compacted collagen gel as a substrate for the growth of corneal cells, particularly limbal corneal epithelial stem cells. Cells grown on such a substrate can be cultured to produce artificial ocular epithelia which can be used in ocular toxicity testing or for transplantation.