Abstract: Disclosed herein is a composition for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a kit for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a method for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples.

Abstract: Disclosed herein are methods for generating an ratiometric symbol for sequential hybridization barcoding for multiplexed Fluorescence In Situ Hybridization (FISH). Also, the disclosure sets forth methods, in addition to using the same, and other solutions to problems in the relevant field.

Abstract: Disclosed herein are methods for generating a massive number of chemically ligateable probes for multiplexed Fluorescence In Situ Hybridization (FISH) using a hybrid-primer. Also, the disclosure sets forth methods, in addition to using the same, and other solutions to problems in the relevant field.

Abstract: Disclosed herein is a composition for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a kit for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a method linked amplification tethered with exponential radiance for signal amplification.

Abstract: This disclosure herein sets forth embodiments to provide a serological test to detect target analytes that can scale to up to 10,000 or more samples in a single run. This disclosure herein sets forth methods to allow for unique barcoding by using a multi-level barcode scheme that is modular and enables easy detection of multiple analytes in samples.

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Pre-designed barcodes are associated specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is developed to overcome limitations in the previous generation of the technology such as lack of visual signals that can be associated with the probes or small internal within cell when carrying out in situ experiments. The current method can be applied to both in vitro and in situ analysis. According to the method, each barcoding round comprises multiple serial hybridizations where a small number of colored signals (that are associated with probes) are used in each hybridization experiment within a serial hybridization round. Images from each serial hybridization experiment within the same serial hybridization round are combined to form a composite image for each barcoding round.

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, nucleic acid probes include a signal moiety connected with a binding sequence via a cleavable linker.