Abstract: The present invention relates to a method for extracting the oleaginous substances from sporoderm-broken Ganoderma spores using SCF-CO2. The method requires: (1) breaking the Ganoderma spores by a mechanical means to obtain the sporoderm-broken spores; and (2) extracting the oleaginous substances from the sporoderm-broken spores using a supercritical fluid-carbon dioxide (SCF-CO2) extraction method. The preferred supercritical conditions include 5 M to 60 M Pa of pressure; 32° C. to 85° C. of temperature; and 5 kg/h to 80 kg/h of flow capacity rate. The total extraction time in SCF-CO2 is between 0.5 hour and 6 hours. The method produces approximately 37% by weight of oleaginous substances from the sporoderm-broken Ganoderma spores. These oleaginous substances are transparent and contain the special fragrance of Ganoderma spores. There is no trace of deposit, solvent residue, or oxidization in the oleaginous substances.

Abstract: The present invention relates to a method for extracting the oleaginous substances from sporoderm-broken Ganoderma spores using SCF—CO2. The method contains the steps of: (1) inducing germination of Ganoderma spores by incubating the spores in a nutritional solution; (2) activating the Ganoderma spores by placing the germination-induced spores in a well ventilated culture box kept at constant temperature and humidity; (3) breaking the Ganoderma spores by a mechanical means to obtain the sporoderm-broken spores; and (4) extracting the oleaginous substances from the sporoderm-broken spores using a supercritical fluid—carbon dioxide (SCF—CO2) extraction method. The preferred supercritical conditions include 5 M to 60 M Pa of pressure; 32° C. to 85° C. of temperature; and 5 kg/h to 80 kg/h of flow capacity rate. The total extraction time in SCF—CO2 is between 0.5 hour to 6 hour.

Abstract: The present invention relates to a method for extracting the oleaginous substances from sporoderm-broken Ganoderma spores using SCF—CO2. The method contains the steps of: (1) inducing germination of Ganoderma spores by incubating the spores in a nutritional solution; (2) activating the Ganoderma spores by placing the germination-induced spores in a well ventilated culture box kept at constant temperature and humidity; (3) breaking the Ganoderma spores by a mechanical means to obtain the sporoderm-broken spores; and (4) extracting the oleaginous substances from the sporoderm-broken spores using a supercritical fluid—carbon dioxide (SCF—CO2) extraction method. The preferred supercritical conditions include 5 M to 60 M Pa of pressure; 32° C. to 85° C. of temperature; and 5 kg/h to 80 kg/h of flow capacity rate. The total extraction time in SCF—CO2 is between 0.5 hour to 6 hour.

Abstract: The present invention describes a method for germination activating spores of red Ganoderma lucidum to produce bioactive substances which has medicinal effects on patients with immunological disorders, cancer, AIDS, hepatitis, diabetes, and cardiovascular diseases, and can prevent or inhibit free radical oxidation and hepatotoxic effects. The method can be subdivided into three stages. At the first stage, a germination activation method is introduced which includes soaking the spores in a solution to induce germination, and placing the germination treated spores in a culture box to induce the synthesis of bioactive substances and softening of the cell walls of the spores. At the second stage, sporoderm-broken ganoderma spores are collected by treating the epispores with cell wall breaking enzymes and/or mechanical force. At the last stage, the bioactive substances are extracted from the sporoderm-broken spores by drying at low temperature followed by extraction.

Abstract: The present invention describes a method for germination activating spores of red Ganoderma lucidum to produce bioactive substances which has medicinal effects on patients with immunological disorders, cancer, AIDS, hepatitis, diabetes, and cardiovascular diseases, and can prevent or inhibit free radical oxidation and hepatotoxic effects. The method can be subdivided into three stages. At the first stage, a germination activation method is introduced which includes soaking the spores in a solution to induce germination, and placing the germination treated spores in a culture box to induce the synthesis of bioactive substances and softening of the cell walls of the spores. At the second stage, sporoderm-broken ganoderma spores are collected by treating the epispores with cell wall breaking enzymes and/or mechanical force. At the last stage, the bioactive substances are extracted from the sporoderm-broken spores by drying at low temperature followed by extraction.