Abstract: The present invention provides a process for enzymatic deinking of office waste paper inclusive of xerographic and inkjet-printed paper printed with non impact and non dispersible ink using the cell free culture supernatant of Vibrio alginolyticus or immobilized cells of the same bacterium having the accession number NIO/DI/32 and deposited at ARS Patent Culture Collection, USA, under the Number NRRL-B-30638. Further, this invention relates to decolorization of office waste paper pulp within 72 h after contacting the said enzymes from the said bacterium in the pulp of 3-12% consistency in seawater. Decolorization is achieved by using extracellular enzymes produced by the bacterium NIO/DI/32 grown in nutrient broth supplemented with starch or Tween 80.