Abstract: Provided is a lentivirus packaging system, which comprises: a transfer plasmid comprising a nucleotide sequence of TAR-reserved-chimeric 5? long terminal repeat (LTR); at least one packaging plasmid comprising a nucleotide sequence encoding TAR RNA binding protein, a nucleotide sequence of rev gene, a nucleotide sequence of gag gene, and a nucleotide sequence of pol gene; and an envelope plasmid. Due to the expression of gene of TAR RNA binding protein by the packaging plasmids, the produced lentivirus has higher virus titer and can improve the transduction rate and the gene delivery efficiency during cell transduction. The present invention further provides a method of improving lentivirus production in a host cell, which comprises using the lentivirus packaging system to transfect the host cell. The present invention further provides a cell transduced by the lentivirus and a method of using the cell for treating cancer.

Abstract: A fan shaft structure includes an annular groove portion, an end head adjacent to the groove portion, and several teeth equally spaced around the surface of the shaft. The annular groove portion, end head and teeth constitute an insert part of the fan shaft, around which the plastic fan blade is molded by insert molding. The present invention enables the center part of the fan blade to enwrap the insert part of the shaft, and firmly engage with the shaft. It is, therefore, not only able to enhance the robustness of the combination but also successful in preserving the adhesion effect between the shaft and the fan blade.

Abstract: A fan shaft structure includes an annular groove portion, an end head adjacent to the groove portion, and several teeth equally spaced around the surface of the shaft. The annular groove portion, end head and teeth constitute an insert part of the fan shaft, around which the plastic fan blade is molded by insert molding. The present invention enables the center part of the fan blade to enwrap the insert part of the shaft, and firmly engage with the shaft. It is, therefore, not only able to enhance the robustness of the combination but also successful in preserving the adhesion effect between the shaft and the fan blade.