Abstract: This disclosure relates to an apparatus and method for sorting motile sperms from crude semen. the enrichment of a population of spermatozoa with capacitated spermatozoa can be achieved by providing a 1-to-4-degree vertical temperature difference in combination with the swim-up method.

Abstract: A sperm sorting device adapted to collaborate with a temperature controlling component is provided. The sperm sorting device includes an upper portion, a lower portion and a porous layer. The upper portion is formed with an upper chamber. The lower portion is formed with a lower chamber. The upper chamber and the lower chamber are disposed vertically. The temperature controlling component is adapted to be disposed outside the upper portion or the lower portion to make the temperature of the upper chamber higher than the lower chamber. The porous layer is disposed between the upper chamber and the lower chamber. The porous layer is adapted to allow sperms to pass through from the lower chamber to the upper chamber and adapted to form a temperature difference between the upper chamber and the lower chamber. Besides, a temperature difference device adapted for the sperm sorting device is also provided.

Abstract: A sperm sorting method is provided. The sperm sorting method includes the following steps: providing a porous layer with a first side and a second side opposite to the first side; disposing an unsorted sperm group at the second side; forming a temperature difference between the first side and the second side; and collecting a sorted sperm group which arrives the first side and passes through the porous layer from the second side based on the temperature difference and overcoming gravity.

Abstract: A microfluidic chip for sorting sperm includes a substrate, a sample channel, and a plurality of divergent channels. The sample channel is disposed in the substrate. The divergent channels are disposed in the substrate. Each of the divergent channels includes a main channel and two branch channels. The two branch channels are connected to an end of the main channel away from the sample channel. The two branch channels are disposed at two sides of the main channels respectively. The sample channel and the main channels of the plurality of divergent channels are substantially connected to each other in serial along a direction.