Abstract: Disclosed is a method to establish an efficient tissue culture system for rapid Aquilaria sinensis propagation. In the present disclosure, tender stem segments of Aquilaria sinensis seedlings or seeds of Aquilaria sinensis harvested in a current year are used as explants. After a plurality of rounds of disinfection, axillary buds are induced to germinate in a sterile environment, and then induced to differentiate into clustered buds, and single buds are then subjected to rooting induction to obtain complete sterile plants. The present disclosure solves the problems of limited ways to acquire materials and slow propagation rate of traditional sowing propagation methods.