Patents by Inventor Cheng Frank Zhong

Cheng Frank Zhong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11387096
    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.
    Type: Grant
    Filed: August 12, 2020
    Date of Patent: July 12, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Shifeng Li, Jian Gong, Yan-You Lin, Cheng Frank Zhong
  • Publication number: 20220134336
    Abstract: Embodiments include systems for sequencing a biological sample. The system may include a reusable subsystem and a removable subsystem. The reusable subsystem may actuate and operate the removable subsystem to automate the sequencing. A base unit of the reusable subsystem may form a fluidic connection between an integrated reagent cartridge and an integrated sensor cartridge of the removable subsystem. The integrated reagent cartridge may be configured to hold reagents and the integrated sensor cartridge may be configured with a biosensor for sequencing the biological sample.
    Type: Application
    Filed: October 25, 2021
    Publication date: May 5, 2022
    Inventors: Sz-Chin Lin, Cheng Frank Zhong, Yiwen Ouyang, Sixing Li, Daqing Liu
  • Patent number: 11255793
    Abstract: Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: February 22, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Cheng Frank Zhong, Shifeng Li
  • Patent number: 11254983
    Abstract: Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice.
    Type: Grant
    Filed: November 16, 2020
    Date of Patent: February 22, 2022
    Assignee: Illumina, Inc.
    Inventors: Dietrich Dehlinger, Cheng Frank Zhong, Juraj Topolancik
  • Patent number: 11221298
    Abstract: Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: January 11, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Cheng Frank Zhong, Shifeng Li
  • Publication number: 20210402391
    Abstract: A microfluidic apparatus (100) can include a PCB (110), a biological chip (120) overlying the PCB (110), and a microfluidic housing (130) overlying the biological chip (120) and the PCB (110). The microfluidic apparatus (100) also has a first adhesive layer (141) attaching the microfluidic housing (130) to the biological chip (120) and a second adhesive layer (142) attaching the microfluidic housing (130) to the PCB (110). The second adhesive layer (142) is thicker than the first adhesive layer (141). The first adhesive layer (141) comprises a first adhesive material, and the second adhesive layer (142) comprises a second adhesive material.
    Type: Application
    Filed: November 15, 2019
    Publication date: December 30, 2021
    Inventors: Liang Wang, Cheng Frank Zhong, Shifeng Li
  • Publication number: 20210399030
    Abstract: A photodiode (112,200,400,500) includes a semiconductor substrate (210) having a first surface (211) and a second surface (212,412,512), and a light sensing junction (220) located adjacent to the first surface (211). The second surface (212,412, 512) is located opposite the first surface (211), and the second surface (212,412,512) includes a concave surface covering a recessed region (415,515) in the semiconductor substrate (210).
    Type: Application
    Filed: November 29, 2019
    Publication date: December 23, 2021
    Inventors: Shifeng Li, Cheng Frank Zhong
  • Publication number: 20210379594
    Abstract: An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.
    Type: Application
    Filed: November 8, 2019
    Publication date: December 9, 2021
    Inventors: Jian Gong, Liang Wang, Yan-You Lin, Cheng Frank Zhong
  • Publication number: 20210381977
    Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.
    Type: Application
    Filed: August 18, 2021
    Publication date: December 9, 2021
    Inventors: Cheng Frank Zhong, Hod Finkelstein, Boyan Boyanov, Dietrich Dehlinger, Darren Segale
  • Patent number: 11181478
    Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: November 23, 2021
    Assignee: ILLUMINA, INC.
    Inventors: Cheng Frank Zhong, Hod Finkelstein, Boyan Boyanov, Dietrich Dehlinger, Darren Segale
  • Publication number: 20210292833
    Abstract: This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.
    Type: Application
    Filed: March 16, 2021
    Publication date: September 23, 2021
    Inventors: Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
  • Patent number: 11125691
    Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: September 21, 2021
    Assignee: ILLUMINA, INC.
    Inventors: Cheng Frank Zhong, Hod Finkelstein, Boyan Boyanov, Dietrich Dehlinger, Darren Segale
  • Publication number: 20210238673
    Abstract: Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second. wavelengths being separated from one another by a first non.-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice.
    Type: Application
    Filed: November 16, 2020
    Publication date: August 5, 2021
    Inventors: Dietrich Dehlinger, Cheng Frank Zhong, Juraj Topolancik
  • Publication number: 20210178397
    Abstract: An apparatus for forming a plurality of microdroplets (26a, 26?, 56) from a droplet (16, 26, 263) includes a substrate (12, 22, 22b, 22c, 1101), a dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43) on the substrate (12, 22, 22b, 22c, 1101) and having a plurality of hydrophilic surface regions (48a, 491, 49) spaced apart from each other by a hydrophobic surface (44, 46), and a plurality of electrodes (14, 14a, 14b, 24, 24a, 24b, 24c, 34a, 34b, 34c) in the dielectric layer (13, 23, 23b, 23c, 43a, 43b, 43).
    Type: Application
    Filed: August 1, 2019
    Publication date: June 17, 2021
    Inventors: Jian GONG, Shifeng LI, Cheng Frank ZHONG
  • Patent number: 11007523
    Abstract: A microfluidic device includes a substrate, a sensor, and one or more lamination films. The top surface of the substrate can include first recessed grooves forming first open channels and the bottom surface of the plastic substrate can include a first recessed cavity and second recessed groves forming second open channels. A first lamination film can be adhered with the top surface of the plastic substrate to form first closed channels. A second lamination film can be adhered to the bottom surface of the plastic substrate to form second closed channels. The sensor can be on the bottom surface of the substrate such that it overlies the first recessed cavity to form a flow cell with the sensor top surface inward facing. A first closed channel can be fluidically connected with a second closed channel and a first or second closed channel can be fluidically connected with the flow cell.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: May 18, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Chen Li, Cheng Frank Zhong, Yu Liu, Yiwen Ouyang
  • Publication number: 20210116372
    Abstract: An integrated detection, flow cell and photonics (DFP) device is provided that comprises a substrate having an array of pixel elements that sense photons during active periods. The substrate and pixel elements form an IC photon detection layer. At least one wave guide is formed on the IC photo detection layer as a photonics layer. An optical isolation layer is formed over at least a portion of the wave guide. A collection of photo resist (PR) walls patterned to define at least one flow cell channel that is configured to direct fluid along a fluid flow path. The wave guides align to extend along the fluid flow path. The flow cell channel is configured to receive samples at sample sites that align with the array of pixel elements.
    Type: Application
    Filed: December 28, 2020
    Publication date: April 22, 2021
    Inventors: Hod Finkelstein, Cheng Frank Zhong, Eliane H. Trepagnier
  • Patent number: 10968482
    Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.
    Type: Grant
    Filed: May 18, 2020
    Date of Patent: April 6, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
  • Publication number: 20210069701
    Abstract: Embodiments of the disclosure include methods and apparatuses for separating beads from a droplet main body on a microfluidics actuator by applying a magnetic field to a droplet disposed at a first location, the droplet including one or more magnetically responsive beads; and moving the magnetic field to separate the one or more magnetically responsive beads from a main body of the droplet. Embodiments also include methods and apparatuses for introducing one or more beads into a droplet main body by applying a magnetic field to one or more magnetically responsive beads and moving the magnetic field to introduce the one or more magnetically responsive beads into a droplet disposed on a first location, wherein the droplet includes a fluid.
    Type: Application
    Filed: September 9, 2020
    Publication date: March 11, 2021
    Inventors: Jian Gong, Yan-You Lin, Sz-Chin Lin, Cheng Frank Zhong
  • Patent number: 10895534
    Abstract: An integrated detection, flow cell and photonics (DFP) device is provided that comprises a substrate having an array of pixel elements that sense photons during active periods. The substrate and pixel elements form an IC photon detection layer. At least one wave guide is formed on the IC photo detection layer as a photonics layer. An optical isolation layer is formed over at least a portion of the wave guide. A collection of photo resist (PR) walls patterned to define at least one flow cell channel that is configured to direct fluid along a fluid flow path. The wave guides align to extend along the fluid flow path. The flow cell channel is configured to receive samples at sample sites that align with the array of pixel elements.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: January 19, 2021
    Assignee: Illumina, Inc.
    Inventors: Hod Finkelstein, Cheng Frank Zhong, Eliane H. Trepagnier
  • Publication number: 20210013025
    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.
    Type: Application
    Filed: August 12, 2020
    Publication date: January 14, 2021
    Inventors: Shifeng Li, Jian Gong, Yan-You Lin, Cheng Frank Zhong