Patents by Inventor Cheng Frank Zhong

Cheng Frank Zhong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240352520
    Abstract: Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
    Type: Application
    Filed: July 3, 2024
    Publication date: October 24, 2024
    Inventor: Cheng Frank Zhong
  • Patent number: 12060606
    Abstract: Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: August 13, 2024
    Assignee: MGI Tech Co., Ltd.
    Inventor: Cheng Frank Zhong
  • Patent number: 12057309
    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.
    Type: Grant
    Filed: June 9, 2022
    Date of Patent: August 6, 2024
    Assignee: MGI Tech Co., Ltd.
    Inventors: Shifeng Li, Jian Gong, Yan-You Lin, Cheng Frank Zhong
  • Patent number: 12043868
    Abstract: This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.
    Type: Grant
    Filed: December 21, 2022
    Date of Patent: July 23, 2024
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
  • Publication number: 20240158836
    Abstract: The technology provided herein relates to high resolution multiplex methods and kits for detecting different analytes in a sample in parallel by sequential signal-encoding of said analytes, wherein the method allows a differentiation of targets which distance is below the diffraction limit of optical microscopes, that is, targets with spatial optical overlap. The disclosed methods also include in vitro methods for screening, identifying and/or testing a substance and/or drug and in vitro methods for diagnosis of a disease, and an optical multiplexing system.
    Type: Application
    Filed: June 29, 2023
    Publication date: May 16, 2024
    Applicant: Resolve Biosciences GmbH
    Inventors: Cheng Frank ZHONG, Christian Korfhage, Frank Reinecke, Stephan Tirier
  • Publication number: 20240076733
    Abstract: This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.
    Type: Application
    Filed: December 21, 2022
    Publication date: March 7, 2024
    Inventors: Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
  • Publication number: 20240066522
    Abstract: An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.
    Type: Application
    Filed: November 7, 2023
    Publication date: February 29, 2024
    Inventors: Jian Gong, Liang Wang, Yan-You Lin, Cheng Frank Zhong
  • Publication number: 20240060890
    Abstract: An integrated detection, flow cell and photonics (DFP) device is provided that comprises a substrate having an array of pixel elements that sense photons during active periods. The substrate and pixel elements form an IC photon detection layer. At least one wave guide is formed on the IC photo detection layer as a photonics layer. An optical isolation layer is formed over at least a portion of the wave guide. A collection of photo resist (PR) walls patterned to define at least one flow cell channel that is configured to direct fluid along a fluid flow path. The wave guides align to extend along the fluid flow path. The flow cell channel is configured to receive samples at sample sites that align with the array of pixel elements.
    Type: Application
    Filed: November 2, 2023
    Publication date: February 22, 2024
    Inventors: Hod Finkelstein, Cheng Frank Zhong, Eliane H. Trepagnier
  • Patent number: 11865543
    Abstract: An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: January 9, 2024
    Assignee: MGI Tech Co., Ltd.
    Inventors: Jian Gong, Liang Wang, Yan-You Lin, Cheng Frank Zhong
  • Patent number: 11841322
    Abstract: An integrated detection, flow cell and photonics (DFP) device is provided that comprises a substrate having an array of pixel elements that sense photons during active periods. The substrate and pixel elements form an IC photon detection layer. At least one wave guide is formed on the IC photo detection layer as a photonics layer. An optical isolation layer is formed over at least a portion of the wave guide. A collection of photo resist (PR) walls patterned to define at least one flow cell channel that is configured to direct fluid along a fluid flow path. The wave guides align to extend along the fluid flow path. The flow cell channel is configured to receive samples at sample sites that align with the array of pixel elements.
    Type: Grant
    Filed: December 28, 2020
    Date of Patent: December 12, 2023
    Assignee: Illumina, Inc.
    Inventors: Hod Finkelstein, Cheng Frank Zhong, Eliane H. Trepagnier
  • Publication number: 20230358678
    Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.
    Type: Application
    Filed: June 21, 2023
    Publication date: November 9, 2023
    Inventors: Cheng Frank Zhong, Hod Finkeslstien, Boyan Boyanov, Dietrich Dehlinger, Darren Segale
  • Patent number: 11719637
    Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.
    Type: Grant
    Filed: August 18, 2021
    Date of Patent: August 8, 2023
    Assignee: ILLUMINA, INC.
    Inventors: Cheng Frank Zhong, Hod Finkelstein, Boyan Boyanov, Dietrich Dehlinger, Darren Segale
  • Publication number: 20230213686
    Abstract: A device for luminescent imaging includes an array of imaging pixels, a photonic structure over the array of imaging pixels, and an array of features over the photonic structure. A first feature of the array of features is over a first pixel of the array of imaging pixels, and a second feature of the array of features is over the first pixel and spatially displaced from the first feature. A first luminophore is within or over the first feature, and a second luminophore is within or over the second feature. The device includes a radiation source to generate first photons having a first characteristic at a first time, and generate second photons having a second characteristic at a second time. The first pixel selectively receives luminescence emitted by the first and second luminophores responsive to the first photons at the first time and second photons at the second time, respectively.
    Type: Application
    Filed: January 10, 2023
    Publication date: July 6, 2023
    Applicant: Illumina, Inc.
    Inventors: Juraj Topolancik, Cheng Frank Zhong
  • Patent number: 11579336
    Abstract: A device for luminescent imaging includes an array of imaging pixels, a photonic structure over the array of imaging pixels, and an array of features over the photonic structure. A first feature of the array of features is over a first pixel of the array of imaging pixels, and a second feature of the array of features is over the first pixel and spatially displaced from the first feature. A first luminophore is within or over the first feature, and a second luminophore is within or over the second feature. The device includes a radiation source to generate first photons having a first characteristic at a first time, and generate second photons having a second characteristic at a second time. The first pixel selectively receives luminescence emitted by the first and second luminophores responsive to the first photons at the first time and second photons at the second time, respectively.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: February 14, 2023
    Assignee: Illumina, Inc.
    Inventors: Juraj Topolancik, Cheng Frank Zhong
  • Patent number: 11560591
    Abstract: This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: January 24, 2023
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
  • Publication number: 20220301848
    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.
    Type: Application
    Filed: June 9, 2022
    Publication date: September 22, 2022
    Inventors: Shifeng Li, Jian Gong, Yan-You Lin, Cheng Frank Zhong
  • Patent number: 11387096
    Abstract: A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.
    Type: Grant
    Filed: August 12, 2020
    Date of Patent: July 12, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Shifeng Li, Jian Gong, Yan-You Lin, Cheng Frank Zhong
  • Publication number: 20220134336
    Abstract: Embodiments include systems for sequencing a biological sample. The system may include a reusable subsystem and a removable subsystem. The reusable subsystem may actuate and operate the removable subsystem to automate the sequencing. A base unit of the reusable subsystem may form a fluidic connection between an integrated reagent cartridge and an integrated sensor cartridge of the removable subsystem. The integrated reagent cartridge may be configured to hold reagents and the integrated sensor cartridge may be configured with a biosensor for sequencing the biological sample.
    Type: Application
    Filed: October 25, 2021
    Publication date: May 5, 2022
    Inventors: Sz-Chin Lin, Cheng Frank Zhong, Yiwen Ouyang, Sixing Li, Daqing Liu
  • Patent number: 11255793
    Abstract: Embodiments of the invention provide an improved biosensor for biological or chemical analysis. According to embodiments of the invention, backside illumination (BSI) complementary metal-oxide-semiconductor (CMOS) image sensors can be used to effectively analyze and measure fluorescence or chemiluminescence of a sample. This measured value can be used to help identify a sample. Embodiments of the invention also provide methods of manufacturing an improved biosensor for biological or chemical analysis and systems and methods of DNA sequencing.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: February 22, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Cheng Frank Zhong, Shifeng Li
  • Patent number: 11254983
    Abstract: Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice.
    Type: Grant
    Filed: November 16, 2020
    Date of Patent: February 22, 2022
    Assignee: Illumina, Inc.
    Inventors: Dietrich Dehlinger, Cheng Frank Zhong, Juraj Topolancik