Abstract: A light module is provided. The light module applied to a dark field microscope is used for illuminating an object. The light module includes a light beam, a reflection component and a condensing component. The light beam has several lights. The reflection component is used for converting the lights radiating along a beginning direction to a circular beam substantially radiating along the beginning direction. The circular beam passes through the condensing component and is focused on the object. A part of the circular beam passing through the condensing component is scattered by the object.

Abstract: A diffraction micro flow structure and optical tweezers using the same are provided. The diffraction micro flow structure comprises a substrate and a diffraction part. The substrate comprises at least a flow path. The diffraction part is disposed on the substrate. The diffraction part comprises a diffraction optical element. After light passes through the diffraction optical element, the light is focused in the flow path and forms an optical field.

Abstract: An optical tweezers controlling device including a light source, an objective lens and a focus adjusting unit is provided. The focus adjusting unit disposed between the light source and the objective lens includes a mirror set and a zoom lens set. The mirror set has at least a mirror. The mirror is rotatable such that after a light of the light source is projected to the mirror, the reflective direction of the light reflected from the mirror is changeable. The zoom lens set has at least a zoom lens disposed in accordance with the mirror. By rotating the mirror or changing the focal length of the zoom lens, the focusing location of the light changes on the focal plane of the objective lens or in the front or the rear of the focal plane.

Abstract: An apparatus and a method for changing optical tweezers are provided. The apparatus includes a diffractive optical element (DOE), a mask unit and an objective lens. The DOE includes a plurality of phase delay patterns. The mask unit includes a plurality of mask patterns that correspond to the phase delay patterns, respectively, wherein at least a portion of the mask patterns are complementary. A laser beam passing through each phase diffractive pattern correspondingly passes through each mask pattern to generate a compound diffractive pattern. The objective lens receives the compound diffractive pattern and focuses it on an examining object to form an optical tweezers.

Abstract: A disk structure is disposed in an optical tweezers device including a light source for producing incident laser light. The disk structure includes a first substrate, a second substrate and a reflective layer. The second substrate is disposed with respect to the first substrate. One of the first substrate and the second substrate has at least one flow path. The reflective layer, which is adhered to the second substrate, is disposed between the first substrate and the second substrate. After the incident laser light passes through the first substrate and then reaches the reflective layer, the incident laser light is reflected back as reflective laser light by the reflective layer to form reflective laser light. A tweezers light field is formed in the flow path by both the reflective laser light and the incident laser light.

Abstract: An apparatus of generating an optical tweezers with momentum and method thereof and an optical tweezers photo-image for guiding particles are provided. The apparatus generates at least one optical tweezers on an examined object that carries at least one particle. The apparatus includes a laser source, a diffractive optical element and a convergent lens. The laser beam from the laser source passes through the diffractive optical element to produce a diffractive pattern. The laser beam is then received by the convergent lens and then to be focused on a plane of the examined object. The optic axis of the convergent lens is substantially not perpendicular to the plane of the examined object, so that the laser beam is projected onto the plane of the examined object in a skewed manner for providing a lateral momentum to move the particle.

Abstract: An apparatus for driving a fluid includes a substrate, at least one electrode group and a controlling unit. The substrate has at least one plane. The electrode group is disposed on the substrate and includes a first electrode, a second electrode and a third electrode. A projecting position of the second electrode on the plane is disposed between that of the first electrode and that of the third electrode. The controlling unit electrically connected to electrode group is for driving the first to third electrodes. When the controlling unit drives the first to third electrodes to make the first and third electrodes have opposite polarities and to make the second and third electrodes have the same polarity, an electric field produced by the electrode group enables the fluid on the substrate to flow from the first electrode to the third electrode.

Abstract: A bubble micro-pump includes a first component, a second component and a bubble-generating unit. The first component includes a flow path having a first area and a second area. The second component above the first component has a first roughness surface and a second roughness surface. The first roughness surface opposite the first area has a first roughness factor, and the second roughness surface opposite the second area has a second roughness factor smaller than the first roughness factor. The bubble-generating unit on the first component generates bubble in the first area and the second area when a fluid fills the vacancy between the first component and the second component. Due to the difference in roughness factor, the backfilling velocity of the fluid in the first area is faster than that in the second area when the bubble starts to vanish, such that the fluid is driven to flow.

Abstract: An apparatus of generating optical tweezers with momentum is provided for providing optical tweezers with a first momentum on a test piece. The apparatus comprises a laser source, a diffractive optical element (DOE) and a lens. The laser source is for outputting a laser beam. The DOE has a first phase-delay picture, and the laser beam forms a diffraction pattern after passing the first phase-delay picture. The lens is for receiving and focusing the diffraction pattern on the test piece to form the first optical tweezers with the first momentum. The lens has an optical axis intersecting the DOE at an optical intersection point, a geometric center of the phase-delay picture has a displacement vector relative to the optical intersection point and a direction of the first momentum is related to a direction of the displacement vector.

Abstract: A method of arranging cells comprises: (a) applying a voltage to two electrodes so as to allow a plurality of cells suspended in a dielectrophoresis-manipulating buffer (DEP-manipulating buffer) to be driven to be arranged into a pattern; (b) replacing the DEP-manipulating buffer with a solution comprising calcium ion and magnesium ion which helps the patterned cells adhere to the substrate; and (c) replacing the solution comprising calcium ion and magnesium ion with a medium so as to allow the patterned cells to grow on the substrate.