Abstract: A physiological signal sensing and compensation system is provided, for contacting an object to be measured and sensing a physiological signal. The physiological signal sensing and compensation system includes a physiological signal sensing module, a compensation module, and a computing unit. The physiological signal sensing module provides an initial sensing signal. The compensation module includes a collecting mechanism for collecting a physiological liquid of the object to be measured. The compensation module provides a compensation signal according to the physiological liquid collected by the collection mechanism. The computing unit is electrically connected to the physiological signal sensing module and the compensation module, and calculates and provides a compensated sensing signal based on the initial sensing signal and the compensation signal.