Patents by Inventor Cheng-Yao Chen

Cheng-Yao Chen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11591629
    Abstract: A method for synthesizing a nucleic acid includes providing an initiator having a 3? end having an unprotected hydroxyl group, providing a nucleic acid polymerase having at least a conservative catalytic polymerase domain of a family-B DNA polymerase, providing a nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase and a metal cofactor which is a bivalent cation, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator. The kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: February 28, 2023
    Inventor: Cheng-Yao Chen
  • Publication number: 20230055565
    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
    Type: Application
    Filed: September 26, 2022
    Publication date: February 23, 2023
    Applicant: Life Technologies Corporation
    Inventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
  • Publication number: 20230037041
    Abstract: An apparatus for preparing nucleic acid sequences using an enzyme, including a reactor, a plurality of nucleotide material bottles, a deblocking material bottle, and a liquid delivering device. The reactor includes a reaction substrate having a pretreated surface. Each of the nucleotide material bottles is adapted to contain a first reaction solution, and the first reaction solution includes a reaction enzyme and a nucleotide having a terminal protecting group. The deblocking material bottle is adapted to contain a deblocking solution. The liquid delivering device is connected to the reactor, the nucleotide material bottles and the deblocking material bottle. The reaction enzyme is adapted to dispose the nucleotide having the terminal protecting group on the pretreated surface. The reactor has an operating temperature of 45° C.-105° C. A method for preparing nucleic acid sequences using an enzyme of the invention is provided.
    Type: Application
    Filed: December 30, 2019
    Publication date: February 2, 2023
    Inventors: Cheng-Yao Chen, Jui-Kang Yen
  • Patent number: 11569236
    Abstract: A method of forming a semiconductor device includes etching a substrate to form two first trenches separated by a fin; filling the two first trenches with an isolation layer; and depositing a dielectric layer over the fin and the isolation layer. The method further includes forming a second trench in the dielectric layer over a channel region of the semiconductor device, the second trench exposing the isolation layer. The method further includes etching the isolation layer through the second trench to expose an upper portion of the fin in the channel region of the semiconductor device, and forming a dummy gate in the second trench over the isolation layer and engaging the upper portion of the fin.
    Type: Grant
    Filed: November 30, 2020
    Date of Patent: January 31, 2023
    Assignee: TAIWAN SEMICONDUCTOR MANUFACTURING COMPANY, LTD.
    Inventors: Hung-Li Chiang, Cheng-Yi Peng, Tsung-Yao Wen, Yee-Chia Yeo, Yen-Ming Chen
  • Publication number: 20220395192
    Abstract: Systems and methods for monitoring sleep using a sleep monitoring system includes a first component and a second component. The first component is configured to be coupled to a chest of the subject and the second component is configured to be concurrently coupled to an abdomen of the subject. Each component includes a housing, a pair of electrode pads mounted on an underside of the respective housing, and an ECG sensor circuit communicatively coupled to the respective pair of electrode pads. The first component further includes a photoplethysmogram sensor that includes at least one light source and at least one photodetector mounted on the underside of the housing of the first component at a location between the first pair of electrode pads.
    Type: Application
    Filed: June 15, 2022
    Publication date: December 15, 2022
    Inventors: Hau-Tieng Wu, Uei-Ming Jow, Cheng-Yao Chen
  • Publication number: 20220376091
    Abstract: In a method of manufacturing a semiconductor device, a fin structure having a channel region protruding from an isolation insulating layer disposed over a semiconductor substrate is formed, a cleaning operation is performed, and an epitaxial semiconductor layer is formed over the channel region. The cleaning operation and the forming the epitaxial semiconductor layer are performed in a same chamber without breaking vacuum.
    Type: Application
    Filed: July 27, 2022
    Publication date: November 24, 2022
    Inventors: Ya-Wen CHIU, Yi Che CHAN, Lun-Kuang TAN, Zheng-Yang PAN, Cheng-Po CHAU, Pin-Ju LIANG, Hung-Yao CHEN, De-Wei YU, Yi-Cheng LI
  • Patent number: 11508710
    Abstract: A method of forming a semiconductor device package includes the following steps. A redistribution structure is formed on a carrier. A plurality of second semiconductor devices are disposed on the redistribution structure. At least one warpage adjusting component is disposed on at least one of the second semiconductor devices. A first semiconductor device is disposed on the redistribution structure. An encapsulating material is formed on the redistribution structure to encapsulate the first semiconductor device, the second semiconductor devices and the warpage adjusting component. The carrier is removed to reveal a bottom surface of the redistribution structure. A plurality of electrical terminals are formed on the bottom surface of the redistribution structure.
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: November 22, 2022
    Assignee: Taiwan Semiconductor Manufacturing Company, Ltd.
    Inventors: Po-Yao Lin, Cheng-Yi Hong, Feng-Cheng Hsu, Shuo-Mao Chen, Shin-Puu Jeng, Shu-Shen Yeh, Kuang-Chun Lee
  • Publication number: 20220356500
    Abstract: A method for preparing nucleic acid sequences using an enzyme, including: (1) providing a reaction substrate having a pretreated surface. (2) Disposing a nucleotide having a terminal protecting group on the pretreated surface by a reaction enzyme, and a reaction temperature is 45° C.-105° C. (3) Removing the terminal protecting group of the nucleotide by irradiation or heating. (4) Coupling another nucleotide having the terminal protecting group to the nucleotide by the reaction enzyme, and a reaction temperature is 45° C.-105° C. (5) Determining whether nucleic acid sequence is completed, and if so, obtaining the nucleic acid sequence, if otherwise repeating steps (3) and (4). The method for preparing nucleic acid sequences using an enzyme of the invention may increase the efficiency of preparing nucleic acid sequences.
    Type: Application
    Filed: December 30, 2019
    Publication date: November 10, 2022
    Inventors: Cheng-Yao Chen, Jui-Kang Yen
  • Publication number: 20220344133
    Abstract: A method for forming a layer includes following operations. A workpiece is received in an apparatus for deposition. The apparatus for deposition includes a chamber, a pedestal disposed in the chamber to accommodate the workpiece, and a ring disposed on the pedestal. The ring includes a ring body having a first top surface and a second top surface and a barrier structure disposed between the first top surface and the second top surface. A vertical distance is defined by a top surface of the barrier structure and a top surface of the workpiece. The vertical distance is between approximately 0 mm and approximately 50 mm. A target disposed in the apparatus for deposition is sputtered. A sputtered material is deposited onto a top surface of the workpiece to form a layer. The barrier structure alters an electrical density distribution during the depositing the sputter material.
    Type: Application
    Filed: July 12, 2022
    Publication date: October 27, 2022
    Inventors: HSIN-LIANG CHEN, WEN-CHIH WANG, CHIA-HUNG LIAO, CHENG-CHIEH CHEN, YI-MING YEH, HUNG-TING LIN, YUNG-YAO LEE
  • Patent number: 11453909
    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: September 27, 2022
    Assignee: Life Technologies Corporation
    Inventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
  • Patent number: 11447756
    Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
    Type: Grant
    Filed: March 20, 2020
    Date of Patent: September 20, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Peter Vander Horn, Cheng-Yao Chen, Guobin Luo, Michael Previte, Jamshid Temirov, Theo Nikiforov, Zhaohui Zhou, Hongye Sun, Yufang Wang, Stefanie Yukiko Nishimura, Hongyi Wang, Marian Peris, Barnett Rosenblum, Michael Phelan
  • Patent number: 11450137
    Abstract: A display device adapted to perform in-screen fingerprint identification is provided. The display device includes a plurality of sub-pixel circuits, a plurality of light sensing circuit stages, and a plurality of sensing drive lines. The light sensing circuit stages correspond to the sub-pixel circuits. The sensing drive lines drive the light sensing circuit stages sequentially. In a first time interval, a first sensing drive line among the sensing drive lines provides a first sensing drive signal to a qth light sensing circuit stage and a latter light sensing circuit stage among the light sensing circuit stages. In the first time interval, the qth light sensing circuit stage performs a light sensing reset operation according to the first sensing drive signal, and the latter light sensing circuit stage performs a light sensing write operation according to the first sensing drive signal.
    Type: Grant
    Filed: January 27, 2021
    Date of Patent: September 20, 2022
    Assignee: Au Optronics Corporation
    Inventors: Ming-Yao Chen, Cheng-Hsing Lin, Shu-Wen Tzeng, Hsin-Lin Hu, Jui-Chi Lo
  • Publication number: 20220195476
    Abstract: A method for nucleic acid synthesis and regeneration of a reusable synthesis initiator includes incorporating a linking nucleotide to an immobilized initiator using a polymerase, synthesizing a nucleic acid right after the linking nucleotide using the polymerase, subjecting a substrate base of the linking nucleotide in the nucleic acid to base-excision by a DNA glycosylase to generate an abasic site, subjecting the abasic site to cleavage by an endonuclease to release the nucleic acid from the initiator, and converting the 3? terminus of the initiator back to its original form by a 3? phosphatase activity-possessing enzyme. A kit based on the aforesaid method and a method for regenerating a reusable initiator are also disclosed.
    Type: Application
    Filed: December 21, 2020
    Publication date: June 23, 2022
    Inventor: Cheng-Yao CHEN
  • Publication number: 20220145272
    Abstract: The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 with mutations in the amino acid sequence positions 409, 410 and 411.
    Type: Application
    Filed: February 13, 2018
    Publication date: May 12, 2022
    Inventors: Jerzy Olejnik, Angela Delucia, Cheng-yao Chen, Ryan Charles Heller, Thomas William Schoenfeld
  • Publication number: 20220127587
    Abstract: The present invention relates to a polymerase enzyme from Pyrococcus furiosus with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 409 of SEQ ID NO. 3: serine (S) (L409S) and/or, (ii) at position 410 of SEQ ID NO. 3: glycine (G) (Y410G) and/or (iii) at position 411 of SEQ ID NO. 3: serine (S) (P411S), wherein the enzyme has little or no 3?-5? exonuclease activity.
    Type: Application
    Filed: February 13, 2018
    Publication date: April 28, 2022
    Inventors: Jerzy Olejnik, Angela Delucia, Cheng-yao Chen, Ryan Charles Heller, Thomas William Schoenfeld
  • Patent number: 11242560
    Abstract: A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: February 8, 2022
    Assignee: Illumina, Inc.
    Inventors: Michael Previte, Molly He, Rigo Pantoja, Cheng-Yao Chen, Chunhong Zhou
  • Patent number: 11198854
    Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
    Type: Grant
    Filed: May 22, 2020
    Date of Patent: December 14, 2021
    Assignee: Illumina, Inc.
    Inventors: Erin Bomati, Michael Previte, Matthew William Kellinger, Cheng-Yao Chen, Molly He
  • Publication number: 20210317424
    Abstract: A method for synthesizing a nucleic acid includes providing an initiator having an unprotected nucleoside base and a 3? hydroxyl group at a 3? terminus, providing a nucleic acid polymerase having at least one conservative catalytic polymerase domain of a family-B DNA polymerase, providing at least one nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase, at a temperature ranging from 20° C. to 90°, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator to form the nucleic acid. Also disclosed is a kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.
    Type: Application
    Filed: June 22, 2021
    Publication date: October 14, 2021
    Inventor: Cheng-Yao CHEN
  • Publication number: 20210246435
    Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
    Type: Application
    Filed: November 20, 2020
    Publication date: August 12, 2021
    Applicant: Illumina, Inc.
    Inventors: Cheng-Yao Chen, Erin Bomati, Molly He
  • Publication number: 20210189447
    Abstract: A method for synthesizing a nucleic acid includes providing an initiator having a 3? end having an unprotected hydroxyl group, providing a nucleic acid polymerase having at least a conservative catalytic polymerase domain of a family-B DNA polymerase, providing a nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase and a metal cofactor which is a bivalent cation, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator. The kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.
    Type: Application
    Filed: December 23, 2019
    Publication date: June 24, 2021
    Inventor: Cheng-Yao CHEN