Abstract: An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiation potential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells.

Abstract: A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability.

Abstract: A method for enhancing differentiation of dopaminergic (DA) neurons is provided. The method of the present invention includes culturing precursor cells; administering urocortin to the precursor cells; and differentiating the precursor cells into the DA neurons.

Abstract: A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability.

Abstract: An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiation potential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells.

Abstract: A novel use of a neuropeptide for treating brain injury in a subject in need thereof is provided. The present invention also provides various amenable routes of administering urocortin, that is, via injection intracerebroventricularly and intraperitoneally, and via intravenous bolus administration.