Abstract: An ammonolysis solution and an ammonolysis method. The ammonolysis solution comprises amine, butanol, amino alcohol, and an alkaline aqueous solution. The ammonolysis method comprises: (1) adding an ammonolysis solution to a solid-phase synthesis column where a solid phase carrier is contained; (2) placing the solid-phase synthesis column into a sealed container where an ammonolysis buffer solution is contained, and keeping the solid-phase synthesis column from contacting with the ammonolysis buffer solution; (3) heating the sealed container, so that the solid phase carrier is under a steam atmosphere of the ammonolysis buffer solution, and then cooling; and (4) taking the solid-phase synthesis column out of the sealed container, treating the solid phase carrier in the solid-phase synthesis column using an eluent, and collecting an effluent liquid. The ammonolysis solution is mild in formula.