Abstract: A multi-objective design method for confronting two-pair primers is achieved by applying a calculation means to assess scores of at least one set of confronting two-pair primers according to a DNA template and a design objective so as to determine an optimal solution from the at least one set of confronting two-pair primers. The design objective includes a fourth objective and a ninth objective. The fourth objective is adapted for calculating an overall difference level by summing each differences level between a melting temperature of one of the primers and a preset melting temperature range. The ninth objective is adapted for calculating a product length difference level by summing each difference between a product length formed by one of primer pairs and a user-defined product length formed by a corresponding one of the primer pairs.

Abstract: A method for designing a multi-objective primer pair is disclosed. The method includes inputting a DNA template fragment, a length of a forward primer, a length of a reverse primer, at least two objectives and optimal values for each of the at least two objectives to a computer system; generating, by the computer system, a plurality of primer pairs according to the DNA template fragment, the length of the forward primer and the length of the reverse primer; and calculating, by the computer system, numerical values of the at least two objectives of each of the plurality of primer pairs and inputting the numerical values of the at least two objectives of each of the plurality of primer pairs to a Pareto Chart tool to obtain at least one primer pair, and taking the primer pair as an optimal solution of the DNA template fragment.

Abstract: A method for forming a semiconductor device structure is provided. The method includes forming a gate dielectric layer over a substrate. The method includes forming a work function metal layer over the gate dielectric layer. The method includes forming a glue layer over the work function metal layer. The glue layer is thinner than the gate dielectric layer. The method includes forming a gate electrode over the glue layer. The gate electrode includes fluorine. The method includes annealing the gate electrode. The fluorine diffuses from the gate electrode into the gate dielectric layer.

Abstract: A method for designing a multi-objective primer pair is disclosed. The method includes inputting a DNA template fragment, a length of a forward primer, a length of a reverse primer, at least two objectives and optimal values for each of the at least two objectives to a computer system; generating, by the computer system, a plurality of primer pairs according to the DNA template fragment, the length of the forward primer and the length of the reverse primer; and calculating, by the computer system, numerical values of the at least two objectives of each of the plurality of primer pairs and inputting the numerical values of the at least two objectives of each of the plurality of primer pairs to a Pareto Chart tool to obtain at least one primer pair, and taking the primer pair as an optimal solution of the DNA template fragment.

Abstract: A health care system including a cloud database, a physiological sensor and a processor is disclosed. The cloud database is configured to store a reference waveform and an abnormal data record. The physiological sensor is configured to detect a detected part of a body and to generate a detected physiological signal. The processor is electrically connected to the cloud database and retrieves the reference waveform from the cloud database. The processor is electrically connected to the physiological sensor to receive the detected physiological signal, converts the detected physiological signal into a physiological waveform, compares the physiological waveform with the reference waveform to determine a frequency error and a peak error therebetween, and generates a driving signal when the frequency error is not smaller than a frequency threshold and the peak error is not smaller than a peak threshold. Advantageously, the efficiency of health management can be improved.

Abstract: A device for measuring psychological signals includes a substrate, a light emitting unit, a photoelectric sensor and a microprocessor. The substrate includes a surface that is divided into an emission area and a reception area. The light emitting unit is arranged in the emission area and includes a plurality of light emitting sections. The plurality of light emitting sections irradiates a plurality of lights with different wavelengths on a predetermined location. The photoelectric sensor is arranged in the reception area, and is capable of detecting a plurality of reflected lights from the predetermined location and generating an electrical signal accordingly. The microprocessor is electrically connected to the photoelectric sensor, receives the electrical signal, and converts the electrical signal into a waveform signal. Thus, the detection accuracy can be improved. As such, the detection accuracy of the psychological condition of the user can be improved.