Abstract: An isobaric stable isotope-containing phosphorylated protein labeling reagent, and a preparation method and application thereof are provided. The protein labeling reagent is a phosphorylated dipeptide organophosphorus reagent labeled by a stable isotope such as deuterium-2, carbon-13, oxygen-18, or nitrogen-15. The preparation method includes: (1) preparation of an isobaric stable isotope-containing amino acid with N-terminal protection, (2) preparation of an isobaric stable isotope-containing amino acid activated ester Fmoc/Boc-R1-NHS with N-terminal protection, (3) preparation of an isobaric stable isotope-containing dipeptide, (4) preparation of an isobaric stable isotope-containing phosphite, (5) preparation of an isobaric stable isotope-containing phosphite dipeptide, and (6) preparation of a stable isotope-labeled N-phosphorylated amino acid activated ester.

Abstract: A color paint formula adjusting system and the method thereof are provided, which allow a user to log onto with a stand-alone or a network, input color paint and adjust it. By computer-aided computing of mix ratio of resin, the color paint is made satisfactory to requirements of physical and chemical properties, with the color unchanged, wherein the color paint is manufactured by mixing a plurality of colored pastes. And colored paste content data and color paint mix ratios are stored in a database. The mixture contents input by the user and the amounts thereof to be added are corrected by color paint formula adjusting system. Using the systems and the methods of the present invention, the user just needs to input the formula ratios and a total amount of the color paint to be compounded, and then content amounts of color paint formulas that satisfying the requirements of physical and chemical properties can be obtained, without repeated attempts or profound experience.