Abstract: Provided are a RIC cell, and a preparation method therefor and application thereof. The present invention particularly relates to the lineage-specific differentiation of a pluripotent or multipotent stem cell, specifically to a RIC cell differentiated from a stem cell, and a preparation method therefor and application thereof.

Abstract: A method for regenerating a humoral immunity system. A pluripotent stem cell is used for expressing a RUNX1 gene, a HOXA9 gene and an LHX2 gene to efficiently obtain B cell seeds after in vitro induction differentiation, and after transplantation, a complete humoral immune system can be reconstructed in an animal in which the humoral immune system is missing. According to the method, an antigen-specific antibody immune response can be realized, a specific high-affinity antibody can be generated against an antigen, and immunological memory can be produced. Meanwhile, the reconstructed immune system is safe, and carries no risk of tumorigenicity.

Abstract: The present invention discloses a method for remolding bone marrow microenvironment in a subject having an impaired bone marrow microenvironment, which comprises implanting a composition comprising isolated mesenchymal stromal cells (MSCs) into the bone marrow cavity of the subject. The method of the present invention successfully remolds the bone marrow microenvironment, recovers the normal hematopoiesis of bone marrow, inhibits/delays the pathological process of leukemia and significantly prolongs the survival period. In addition, the method according to the present invention can be used for the treatment of hematologic tumors such as leukemia and aplastic anemia (AA), which is safe and effective but has no side effects.

Abstract: The present invention discloses a method for remolding bone marrow microenvironment in a subject having an impaired bone marrow microenvironment, which comprises implanting a composition comprising isolated mesenchymal stromal cells (MSCs) into the bone marrow cavity of the subject. The method of the present invention successfully remolds the bone marrow microenvironment, recovers the normal hematopoiesis of bone marrow, inhibits/delays the pathological process of leukemia and significantly prolongs the survival period. In addition, the method according to the present invention can be used for the treatment of hematologic tumors such as leukemia and aplastic anemia (AA), which is safe and effective but has no side effects.