Abstract: This work provides a method and device for performing quantitative and sensitive multiplex nucleic acid detection at the point-of-care using magnetoresistive (MR) detection. Temperature calibration of the MR sensor elements is performed per-element, rather than assuming the same calibration parameters apply to each element of the MR sensor array. It can include a digitally controlled fluidic system to allow automated wash and reagent injection, an on-chip temperature management system to achieve on-chip polymerase chain reactions (PCR), and a portable magnetoresistive sensor platform. This approach requires minimal user involvement beyond adding the sample and simple top-level control, making it highly desirable for point-of-care applications.