Patents by Inventor Chenhui WEN

Chenhui WEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250171816
    Abstract: The present invention relates to an enzyme combination, genetically engineered bacteria, and application thereof in producing D-psicose. Glucose isomerase and D-psicose 3-epimerase from specific sources are separately co-expressed in Bacillus subtilis, a combination enzyme containing glucose isomerase and D-psicose 3-epimerase is obtained through fermentation, and the combination enzyme is used to catalyze a glucose substrate to perform isomerization to eventually prepare D-psicose. The method provided in the present invention can clearly increase a conversion rate of D-psicose, which greatly reduces the time of converting glucose into D-psicose. In addition, inexpensive glucose is used as a raw material, so that the production costs of D-psicose are greatly reduced. In the present invention, fructose syrup may be synchronously produced, so that the input for a whole set of production lines of fructose syrup is omitted, thereby greatly improving the economic benefits.
    Type: Application
    Filed: January 30, 2025
    Publication date: May 29, 2025
    Inventors: Sanying WANG, Ziheng JIN, Yanjun WEN, Linzheng LI, Chenhui WEN, Rongrong XIE, Tianyi PAN
  • Publication number: 20250171817
    Abstract: The present invention relates to an enzyme combination, genetically engineered bacteria, and application thereof in producing D-psicose. In the present invention, glucose isomerase and D-psicose 3-epimerase from specific sources are co-expressed in Bacillus subtilis. A crude enzyme preparation is obtained by fermenting obtained engineered bacteria, and isomerization is performed by using high-concentration glucose as a substrate. Fructose syrup and D-psicose syrup are obtained by performing isolation, purification, and concentration. The enzyme combination provided in the present invention has a high catalytic rate for a substrate, and can clearly increase a conversion rate of D-psicose. In addition, inexpensive glucose is used as a raw material, so that the production costs of D-psicose are greatly reduced.
    Type: Application
    Filed: January 30, 2025
    Publication date: May 29, 2025
    Inventors: Sanying WANG, Ziheng JIn, Yanjun Wen, Linzheng Li, Chenhui Wen, Rongrong Xie, Tianyi Pan
  • Publication number: 20220411833
    Abstract: Disclosed are gene engineering bacteria for producing L-arginine and a construction method and an application of the gene engineering bacteria. According to the method, genes encoding a carbamoyl phosphate synthetase and a gene encoding an L-arginine biosynthesis pathway enzyme are integrated into Escherichia coli; the present invention has analyzed and reconstructed the arginine synthetic pathway and the metabolic flow related to arginine in the entire amino acid metabolic network in E. coli and finally obtained a genetically engineered bacterial strain which has a clear genetic background, carries no plasmids, undergoes no mutagenesis and is capable of stably and efficiently producing L-arginine.
    Type: Application
    Filed: May 15, 2020
    Publication date: December 29, 2022
    Inventors: Xixian XIE, Shuai JIANG, Chenhui WEN, Heyun WU, Yining LIU, Xuan LI, Daoguang TIAN, Bo XIONG