Abstract: Provided are an imaging method and apparatus, and a medium. The method includes: for a target field of view, performing photographing and quantification at a first resolution, to obtain a first image that has a first bit width; for the target field of view, performing photographing and differential processing at a second resolution, to obtain a second image that has a second bit width, wherein the differential processing includes: for a pixel point obtained by photographing at the second resolution, quantifying the difference between the pixel point and a neighboring pixel point of the pixel point, to obtain a quantified difference a value of a corresponding pixel point in the second image; and fusing the first image with the second image, to obtain a third image, wherein the first resolution is lower than the second resolution, and the first bit width is higher than the second bit width.

Abstract: A Saccharomyces cerevisiae expression system and a construction method and application thereof, including an expression vector which includes, from 5? to 3?, a YEplac195 plasmid backbone, an exogenous gene expression cassette, and a selective marker gene expression cassette. The exogenous gene expression cassette includes from upstream to downstream an rDNA promoter, an internal ribosome entry site (IRES) sequence, an exogenous gene expression cassette, a poly(T) sequence, and an rDNA terminator. The selective marker gene expression cassette includes a promoter, a selective marker gene, and a transcription terminator.