Patents by Inventor Chi-Huey Wong

Chi-Huey Wong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS AND COMPOSITIONS FOR IMMUNIZATION AGAINST VIRUS
    Publication number: 20150335728
    Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.
    Type: Application
    Filed: February 18, 2014
    Publication date: November 26, 2015
    Applicant: Academia Sinica
    Inventors: Chi-Huey WONG, Che MA, Cheng-Chi WANG, Juine-Ruey CHEN
  • REACTIVE LABELLING COMPOUNDS AND USES THEREOF
    Publication number: 20150309041
    Abstract: Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.
    Type: Application
    Filed: April 28, 2015
    Publication date: October 29, 2015
    Inventors: Chi-Huey WONG, Jim-Min FANG, Jiun-Jie SHIE
  • GLOBO H AND RELATED ANTI-CANCER VACCINES WITH NOVEL GLYCOLIPID ADJUVANTS
    Publication number: 20150273034
    Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.
    Type: Application
    Filed: April 1, 2015
    Publication date: October 1, 2015
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Alice L. Yu, John Yu
  • BENZOCYCLOOCTYNE COMPOUNDS AND USES THEREOF
    Publication number: 20150241439
    Abstract: Provided are benzocyclooctyne compounds of formula (I). These compounds undergo strain-promoted azide-alkyne cyclo additions (SPAAC) without presence of toxic metal catalysts. The provided compounds are useful for diagnosis and imaging of azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.
    Type: Application
    Filed: August 21, 2013
    Publication date: August 27, 2015
    Inventors: Chi-Huey Wong, Jim-Min Fang, Jiun-Jie Shie
  • CRYSTAL STRUCTURE OF BIFUNCTIONAL TRANSGLYCOSYLASE PBP1B FROM E. COLI AND INHIBITORS THEREOF
    Publication number: 20150232417
    Abstract: Crystal structure at 2.16 ? resolution of full-length Escherichia coli penicillin-binding protein 1b (PBP1b) in complex with its inhibitor moenomycin, is provided. 3D structures of amino acid residues involved in moenomycin binding and transglycosylation activity are identified. Binding sites for peptidoglycan synthesis inhibitors comprising amino acid residues from transglycosylase (TG), UvrB domain 2 homolog (UB2H) and transmembrane (TM) domains of PBP1b are identified at atomic level resolution. Rational drug design, based on the atomic coordinates, are disclosed. Methods for screening for antibiotics using anisotropic binding and transglycosylase inhibitor assays and novel antibiotics based on the screening assays are provided.
    Type: Application
    Filed: February 24, 2014
    Publication date: August 20, 2015
    Applicant: ACADEMIA SINICA
    Inventors: Chi-Huey WONG, Che Alex MA, Ting-Jen Rachel CHENG, Wei-Chieh CHENG
  • CELL-PERMEABLE PROBES FOR IDENTIFICATION AND IMAGING OF SIALIDASES
    Publication number: 20150225766
    Abstract: Provided herein are novel irreversible sialidase inhibitors. These compounds can be conjugated with a detectable tagging moiety such as azide-annexed biotin via CuAAC for isolation and identification of sialidases. The provided compounds and the corresponding detectable conjugates are useful for detecting sialidase-containing pathogens and imaging in situ sialidase activities under physiological conditions.
    Type: Application
    Filed: August 16, 2013
    Publication date: August 13, 2015
    Inventors: Chi-Huey Wong, Jim-Min Fang, Yih-Shyun E. Cheng, Charng-Sheng Tsai
  • Compounds and methods for treating tuberculosis infection
    Patent number: 9073941
    Abstract: The present invention provides compounds which are potent inhibitors against Lpd activity, PDH activity, and/or the growth of tubercle bacillus, and thus are useful in the treatment of tuberculosis infection and associated conditions. The present invention is further directed to in vitro- and in vzivo-based methods of inhibiting Lpd and/or PDH activity. In certain embodiments, these methods are useful in inhibiting Lpd and/or PDH activity key to a pathogen's survival.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: July 7, 2015
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Ying-Ta Wu
  • GLYCAN ARRAYS FOR HIGH THROUGHPUT SCREENING OF VIRUSES
    Publication number: 20150160217
    Abstract: Glycan arrays that can detect and distinguish between various sub-types and strains of influenza virus are provided. Methods for using the glycan arrays with assays using nanoparticle amplification technique are disclosed. Sandwich assays using gold nanoparticles conjugated to phage particles comprising influenza virus-specific antibodies for detecting multiple serotypes using a single reaction are provided. Plurality of glycans directed to specific target HA of influenza virus comprises the array. Detector molecules comprising noble metals conjugated to (a) phage display particles expressing antibodies against hemagglutinin and (b) neuraminidase binding agents are disclosed.
    Type: Application
    Filed: April 12, 2011
    Publication date: June 11, 2015
    Applicant: Academia Sinica
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Chi-Hui Liang, An-Suei Yang
  • Globo H and related anti-cancer vaccines with novel glycolipid adjuvants
    Patent number: 9028836
    Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.
    Type: Grant
    Filed: August 7, 2012
    Date of Patent: May 12, 2015
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Alice L. Yu, John Yu
  • QUANTITATIVE ANALYSIS OF CARBOHYDRATE-PROTEIN INTERACTIONS USING GLYCAN MICROARRAYS: DETERMINATION OF SURFACE AND SOLUTION DISSOCIATION CONSTANTS
    Publication number: 20150094237
    Abstract: A method, system and device to identify, study and/or mimic carbohydrate-protein interactions on cell surfaces and in solution measured by a glycan microarray. In some instances the method, system and device uses very small quantities of carbohydrate as low as attomol. In some instances the system, method and device is high-throughput. The small quantity senstivity may allow for close placement of carbohydrate array members wherein due to close proximity multivalent interactions with proteins may be identified.
    Type: Application
    Filed: December 9, 2014
    Publication date: April 2, 2015
    Inventors: Pi-Hui LIANG, Chi-Huey WONG
  • HUMAN iNKT CELL ACTIVATION USING GLYCOLIPIDS WITH ALTERED GLYCOSYL GROUPS
    Publication number: 20150071960
    Abstract: Glycosphingolipids (GSLs) bearing ?-glucose (?-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with ?-glucose (?-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with ?-galactose (?-Gal) are disclosed. GSLs bearing ?-glucose (?-Glc) and derivatives of ?-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with ?-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with ?-Glc and derivatives thereof are provided.
    Type: Application
    Filed: September 8, 2014
    Publication date: March 12, 2015
    Inventors: Chi-Huey WONG, Alice L. Yu, Kun-Hsien Lin, Tai-Na Chen
  • Antibiotic compositions and related screening methods
    Patent number: 8916540
    Abstract: Moenomycin inhibits bacterial growth by clocking the transglycosylase activity of class A penicillin-binding proteins (PBPs), which are key enzymes in bacterial cell wall synthesis. The binding affinities of moenomycin A with various truncated PBPs were compared showing that the transmembrane domain is important for moenomycin binding. Full-length class-A PBPs from 16 bacterial species were produced, and their binding activities showed a correlation with the antimicrobial activity of moenomycin against Enterococcus faecalis and Staphylococcus aureus. Moreover, a fluorescence anisotropy-based high-throughput assay was developed and used successfully for identification of transglycosylase inhibitors.
    Type: Grant
    Filed: January 15, 2009
    Date of Patent: December 23, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Ting-Jen Cheng, Che Alex Ma, Wei-Chieh Cheng
  • Quantitative analysis of carbohydrate-protein interactions using glycan microarrays: determination of surface and solution dissociation constants
    Patent number: 8906832
    Abstract: A method, system and device to identify, study and/or mimic carbohydrate-protein interactions on cell surfaces and in solution measured by a glycan microarray. In some instances the method, system and device uses very small quantities of carbohydrate as low as attomol. In some instances the system, method and device is high-throughput. The small quantity sensitivity may allow for close placement of carbohydrate array members wherein due to close proximity multivalent interactions with proteins may be identified.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: December 9, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Pi-Hui Liang
  • Fungal immunostimulatory compositions
    Patent number: 8906380
    Abstract: Methods are disclosed which are useful in increasing maturation of dendritic cells from CD14+ mononuclear cells, by contact with a composition comprising a fucose-containing glycoprotein fraction from Ganoderma lucidum. The extract can also be used for increasing production of a cytokine or a chemokine in a dendritic cell or CD19+ B cell. In addition, a fucose-containing glycoprotein fraction from Ganoderma lucidum can be administered to a subject identified as needing increased immunoglobulin, cytokine, or chemokine production.
    Type: Grant
    Filed: October 13, 2006
    Date of Patent: December 9, 2014
    Assignee: Academia Sinica
    Inventors: Alice Yu, John Yu, Kuo-I Lin, Wen-Bin Yang, Chi-Huey Wong
  • Method of preparing glycopeptides
    Patent number: 8816050
    Abstract: A method is provided for the synthesis of glycopeptides using a sugar assisted ligation strategy, wherein an N-terminal peptide portion in the form of a thioester is coopled with a C-terminal peptide portion bearing a carbohydrate moiety comprising a thiol group.
    Type: Grant
    Filed: March 22, 2007
    Date of Patent: August 26, 2014
    Inventors: Chi-Huey Wong, Ashraf Brik, Yu-Ying Yang, Simon Ficht, Richard Payne
  • Methods and compositions for preparation of selectively protected carbohydrates
    Patent number: 8802401
    Abstract: The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharides
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: August 12, 2014
    Assignees: The California Institute of Technology, The Scripps Research Institute
    Inventors: Frances H. Arnold, Chi-Huey Wong, Yuuichi Mitsuda, Michael M. Chen, Clay Bennett, William Greenberg, Jared Crawford Lewis, Sabine Bastian
  • GLYCOLIPIDS AND ANALOGUES THEREOF AS ANTIGENS FOR NKT CELLS
    Publication number: 20140186378
    Abstract: This invention relates to immunogenic compounds which serve as ligands for NKT (natural killer T) cells and to methods of use thereof in modulating immune responses.
    Type: Application
    Filed: October 22, 2013
    Publication date: July 3, 2014
    Applicants: THE SCRIPPS RESEARCH INSTITUTE, THE ROCKEFELLER UNIVERSITY
    Inventors: Moriya TSUJI, David D. HO, Chi-Huey WONG, Douglass WU, Masakazu FUJIO, Xiangming LI
  • Methods and compositions for immunization against virus
    Patent number: 8741311
    Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: June 3, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Che Ma, Cheng-Chi Wang, Juine-Ruey Chen
  • Glycan arrays on PTFE-like aluminum coated glass slides and related methods
    Patent number: 8680020
    Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.
    Type: Grant
    Filed: July 15, 2009
    Date of Patent: March 25, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng
  • LARGE SCALE ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES
    Publication number: 20140051127
    Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 20, 2014
    Applicant: Academia Sinica
    Inventors: Chi-Huey Wong, Tsung-I Tsai, Chung-Yi Wu