Abstract: Provided is a nucleic acid purification device which is rotatably installed such that the entire processes of purifying a cell-free nucleic acid from a large amount of blood and a large amount of body fluid are integrated, the nucleic acid purification device including a disk in which a fluid is transferred by a centrifugal force, a supply portion installed in the disk and configured to supply a specimen and a reagent required for nucleic acid purification, an adsorption reaction chamber which is installed in the disk and is connected with the supply portion and in which an adsorption medium for adsorbing a nucleic acid is accommodated and the nucleic acid is adsorbed from the specimen, and a solution accommodating portion which is connected with an output side of an adsorption reaction portion along a centrifugal direction of the disk and in which a solution discharged through the adsorption reaction chamber by the centrifugal force is accommodated and is discharged to the outside.