Abstract: An isolated or a purified nucleic acid sequence for enhancing expression levels of a protein of interest, in 5? to 3? direction, comprises: a cytomegalovirus (CMV) promoter; an eEF-1 intron repeat (eEF-1 IR); and a regular sequence, which comprises: at least one tag element, a fixable linker sequence, wherein the fixable sequence is TEV sequence; and a multiple cloning site (MCS). By means of the array of the specific promoter and eEF-1 IR, the expression and purity of the recombinant protein could be enhanced; wherein eEF-1 IR can reduce the length of vector and assist RNA polymerase II transcription. Besides, TEV sequence of the fixable linker sequence of the regular sequence can remove a tag on recombinant protein; a specific target can be inserted into the multiple cloning site.

Abstract: An isolated or a purified nucleic acid sequence for enhancing expression levels of a protein of interest, in 5? to 3? direction, comprises: a cytomegalovirus (CMV) promoter; an eEF-1 intron repeat (eEF-1 IR); and a regular sequence, which comprises: at least one tag element, a fixable linker sequence, wherein the fixable sequence is TEV sequence; and a multiple cloning site (MCS). By means of the array of the specific promoter and eEF-1 IR, the expression and purity of the recombinant protein could be enhanced; wherein eEF-1 IR can reduce the length of vector and assist RNA polymerase II transcription. Besides, TEV sequence of the fixable linker sequence of the regular sequence can remove a tag on recombinant protein; a specific target can be inserted into the multiple cloning site.

Abstract: A clamp seat of workbench is composed of a seat body, a bearing plate and an elastic clamp. The seat body is of a U-shaped construction and is provided with a plurality of locating holes. The bearing plate is provided with a control handle having a retaining block. The elastic clamp is located in an axial hole of the control handle and is provided with a locating block capable of engaging the locating holes of the seat body so as to set the locating angle of the bearing plate.