Abstract: A manufacturing method of a lithium battery cell is provided and has steps of providing an bag, electrode sheets and a conduit, a part of an edge of the bag being pressed to form an sealing edge, an electrode chamber being enclosed in the bag, the electrode sheet being accommodated in the electrode chamber, the sealing edge being strip-shaped and an air chamber being formed therein, the air chamber being communicated to the electrode chamber, the conduit being disposed on the sealing edge, two ends of the conduit being an inner end and an outer end, the inner end being communicated to the air chamber and separated from the electrode chamber, and the outer end being exposed from the bag; injecting an electrolyte into the electrode chamber through the conduit; pressing the air chamber to form a secondary sealing edge; cutting the secondary sealing edge to remove the conduit.