Abstract: A method for manufacturing a measuring kit is disclosed. The method for manufacturing a measuring kit includes steps of providing a carrier including a substrate having a surface; coating a silane onto the surface of the substrate; providing an organic molecule; adding the organic molecule onto the carrier; outputting a microwave energy by a coaxial cable; and emitting the microwave energy through a radiator to microwave the carrier and the organic molecule uniformly to coat the organic molecule onto the carrier carrying the silane, wherein the measuring kit is used to apply an enzyme-linked immunosorbent assay.

Abstract: A sensing method is disclosed. The sensing method includes steps of providing a carrier including a hole having a bottom, wherein a plurality of spaced apart first nanoparticles are disposed on the bottom; coating a sensing molecule in the hole; providing a testing solution having a testing parameter to the hole, wherein the testing solution has a complex including a testing molecule and a second nanoparticle, and a specific binding occurs between the testing molecule and the sensing molecule; centrifuging the carrier to subside the complex; washing the hole; and measuring a synthetic spectral signal change of the first nanoparticle and the second nanoparticle according to a degree of the specific binding between the testing molecule and the sensing molecule to determine the testing parameter of the testing solution.

Abstract: A method for manufacturing a measuring kit is disclosed. The method for manufacturing a measuring kit includes steps of providing a carrier including a substrate having a surface; coating a silane onto the surface of the substrate; providing an organic molecule; adding the organic molecule onto the carrier; outputting a microwave energy by a coaxial cable; and emitting the microwave energy through a radiator to microwave the carrier and the organic molecule uniformly to coat the organic molecule onto the carrier carrying the silane, wherein the measuring kit is used to apply an enzyme-linked immunosorbent assay.

Abstract: A sensing chip for sensing a first organic molecule in a sample is disclosed. The sensing chip includes a substrate, wherein there are a plurality of separated nanometers on the substrate and a plurality of local substrate surfaces among the plurality of separated nanometers; a silane fixed on the plurality of local substrate surfaces; and a second organic molecule fixed on surfaces of the separated nanometers, wherein there is specificity between the second organic molecule and the first organic molecule.