Abstract: A mental and physical condition estimation system according to the present disclosure includes: a heart rate information acquisition unit for acquiring heart rate information that is information related to a heart rate of a subject person; a heart rate variability calculation unit for calculating heart rate variability of a very low frequency component (VLF) from the acquired heart rate information; and a mental and physical condition estimation unit for estimating a concentration and effort state of the subject person from a calculated value of the heart rate variability.

Abstract: A physiological state index calculation system, a physiological state index calculation method, and a non-transitory computer readable medium for capturing subtle changes in a physiological state of a living body are provided. The physiological state index calculation system includes a band-pass filter that filters cerebral blood flow waveform information obtained from a cerebral blood flow of a living body in at least one frequency band, and a complex number conversion unit configured to convert the filtered cerebral blood flow waveform information into a complex number for at least one frequency band. The cerebral blood flow waveform information converted into a complex number by the complex number conversion unit is an oscillator that reflects a physiological state of a living body.

Abstract: A physiological state index calculation system, a physiological state index calculation method, and a non-transitory computer readable medium for capturing subtle changes in a physiological state of a living body are provided. The physiological state index calculation system includes a band-pass filter that filters cerebral blood flow waveform information obtained from a cerebral blood flow of a living body in at least one frequency band, and a complex number conversion unit configured to convert the filtered cerebral blood flow waveform information into a complex number for at least one frequency band. The cerebral blood flow waveform information converted into a complex number by the complex number conversion unit is an oscillator that reflects a physiological state of a living body.

Abstract: A mental and physical condition estimation system according to the present disclosure includes: a heart rate information acquisition unit for acquiring heart rate information that is information related to a heart rate of a subject person; a heart rate variability calculation unit for calculating heart rate variability of a very low frequency component (VLF) from the acquired heart rate information; and a mental and physical condition estimation unit for estimating a concentration and effort state of the subject person from a calculated value of the heart rate variability.

Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Abstract: A method for producing an expression product of an exogenous gene in a yeast, including using at least one yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.

Abstract: A method for producing an expression product of an exogenous gene in a yeast, including using at least one yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.

Abstract: Provided is a method for producing an expression product of an exogenous gene in a yeast with superior output regulation of gene expression. This is a method for producing an expression product of an exogenous gene in a yeast, including using one or two or more kinds of yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.

Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Abstract: The saccharification efficiency of cellulase is enhanced within reaction temperature regions of general fermenting microorganisms other than heat-resistant yeast. Cellulosic biomass is saccharified with cellulase in the presence of a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2 or 4 and having a function of enhancing the saccharifying activity of cellulase.

Abstract: Provided is a method for producing an expression product of an exogenous gene in a yeast with superior output regulation of gene expression. This is a method for producing an expression product of an exogenous gene in a yeast, including using one or two or more kinds of yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.

Abstract: The saccharification efficiency of cellulase is enhanced within reaction temperature regions of general fermenting microorganisms other than heat-resistant yeast. Cellulosic biomass is saccharified with cellulase in the presence of a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2 or 4 and having a function of enhancing the saccharifying activity of cellulase.

Abstract: A cellobiohydrolase that can contribute to a synergistic effect on cellulose degradation and the use of such cellobiohydrolase in cellulose degradation are provided. The synergistic effect is achieved by an enzyme preparation for cellulose degradation containing a cellobiohydrolase originating in Phanerochaete chrysosporium and belonging to GHF6 or a variant thereof, and an endoglucanase originating in a different source other than Phanerochaete chrysosporium.

Abstract: The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.

Abstract: A cellobiohydrolase that can contribute to a synergistic effect on cellulose degradation and the use of such cellobiohydrolase in cellulose degradation are provided. The synergistic effect is achieved by an enzyme preparation for cellulose degradation containing a cellobiohydrolase originating in Phanerochaete chrysosporium and belonging to GHF6 or a variant thereof, and an endoglucanase originating in a different source other than Phanerochaete chrysosporium.

Abstract: The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.

Abstract: The surface of oxidase is stabilized by stabilizing the inner structure responsible for the enzyme function of the oxidase via the alteration of unstable amino acids therein into stable amino acids, and/or immobilizing the oxidase in a structure unit with structural stability and of a given inner diameter. The inner structure of oxidase is stabilized by altering specific unstable amino acids positioned within a 12-angstrom radius range from the active center of the oxidase or altering specific unstable amino acids facing the hydrogen peroxide-binding pocket of the oxidase. The process enhances the stability of the oxidase, particularly the stability of oxidase with lignin degradation activity against hydrogen peroxide.

Abstract: A method for protein synthesis for efficiently preparing a protein with intermolecular and/or intramolecular disulfide bonds synthetically, using a cell-free extract solution to which a template gene is added. The protein with intermolecular and/or intramolecular disulfide bonds is preferably an antibody or a metalloprotein. The method more preferably includes adding a protein with the chaperone function to the cell-free extract solution and/or eliminating reducing agents from the cell-free extract solution. A method for protein screening and a retrieval method for protein function, using this method for protein synthesis. The method provides a method for protein synthesis capable of efficiently preparing a protein with intermolecular and/or intramolecular disulfide bonds synthetically by cell-free systems (in vitro transcription/translations systems).