Abstract: The present invention provides a method for rapidly clearing a biological tissue. According to the present invention, provided is a method for clearing a biological tissue, including the steps of: infiltrating the biological tissue with water-soluble ethylenically unsaturated monomers before, during or after fixing the biological tissue with a fixative, wherein the water-soluble ethylenically unsaturated monomers include at least a water-soluble ethylenically unsaturated monomer having an ionically dissociable group; polymerizing the water-soluble ethylenically unsaturated monomers to form a hydrogel in the fixed biological tissue; and removing a lipid from the fixed biological tissue.

Abstract: A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.

Abstract: A cell culture container is provided which is filled with a cell culture substrate (14) including a gel layer formed of a gel (15) capable of being denatured by heating and a gold nanoparticle layer (13) formed on one surface of the gel layer, in which a cell is cultured on a side of the one surface or a side of the other surface in the cell culture substrate. A method for manufacturing a cell culture container, including: a step of filling the cell culture container with a gel capable of being denatured by heating; and a step of forming a gold nanoparticle layer on one surface of the gel. A method for acquiring a cell, including: a step of selecting a cell to be acquired from cells placed in the cell culture container; a step of irradiating a cell culture substrate in the vicinity of the selected cell with light; and a step of recovering the selected cell.

Abstract: A cell culture container may include an inlet through which a fluid is supplied, an outlet through which a fluid is discharged, and a flow path configured to connect the inlet to the outlet and accommodate a cell culture substrate containing gold nanoparticles and capable of being denatured by heating.

Abstract: The present invention provides a method for rapidly clearing a biological tissue. According to the present invention, provided is a method for clearing a biological tissue, including the steps of: infiltrating the biological tissue with water-soluble ethylenically unsaturated monomers before, during or after fixing the biological tissue with a fixative, wherein the water-soluble ethylenically unsaturated monomers include at least a water-soluble ethylenically unsaturated monomer having an ionically dissociable group; polymerizing the water-soluble ethylenically unsaturated monomers to form a hydrogel in the fixed biological tissue; and removing a lipid from the fixed biological tissue.

Abstract: A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.