Abstract: The present disclosure relates to a recombinant protein comprising a plurality of type II cohesin repeats. The present disclosure provides a recombinant cellulosome complex comprising: the recombinant protein comprising a plurality of type II cohesin repeats; a recombinant cellulosome complex integrating protein A comprising a plurality of type I cohesin repeats, a plurality of cellulose-binding modules and a type II dockerin; and a plurality of recombinant enzymes each comprising a type I dockerin. A cell, a method for digesting a cellulose and a method for producing ethanol are also provided.

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Abstract: The present disclosure relates to a recombinant protein comprising a plurality of type II cohesin repeats. The present disclosure provides a recombinant cellulosome complex comprising: the recombinant protein comprising a plurality of type II cohesin repeats; a recombinant cellulosome complex integrating protein A comprising a plurality of type I cohesin repeats, a plurality of cellulose-binding modules and a type II dockerin; and a plurality of recombinant enzymes each comprising a type I dockerin. A cell, a method for digesting a cellulose and a method for producing ethanol are also provided.

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Abstract: The present invention provides a method for increasing the metabolic rate of recombinant microorganism growth under an anaerobic environment, wherein a recombinant strain is placed under an anaerobic environment and cultured under a culture condition, wherein the culture condition includes a potential difference and a nitrogen source, but not includes an organic carbon source. According to the method disclosed by the present invention, the recombinant strain can perform anaerobic respiration and metabolic reaction in an anaerobic environment, and can grow stably and rapidly.

Abstract: Described herein is an engineered Kluyveromyces marxianus cell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.

Abstract: Described herein is an engineered Kluyveromyces marxianus cell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

Abstract: Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5? end of the nucleic acid molecule, and (b) an overlapping sequence at the 3? end of the nucleic acid molecule.

Abstract: Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5? end of the nucleic acid molecule, and (b) an overlapping sequence at the 3? end of the nucleic acid molecule.

Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.

Abstract: An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.

Abstract: A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.

Abstract: An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.

Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.

Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.

Abstract: A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.

Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.