Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

Abstract: The present invention provides a DNA encoding a raffinose synthase from soybean and vectors containing the same. Also provided are plants and host cells transformed with the DNA sequence and methods of altering the levels of raffinose family oligosaccharides in plants.

Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

Abstract: Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

Abstract: Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

Abstract: A method for producing an L-amino acid, which comprises inserting a gene which codes for an enzyme which is utilized on the route of biosynthesis of an L-amino acid product into one of at least two plasmid vectors which have compatible replicating origins different from each other, inserting a second gene which codes for an enzyme different from the first enzyme on the route of biosynthesis of the L-amino acid into a second of the plasmid vectors; introducing the thus obtained recombinant plasmids into a strain of Coryneform bacteria; and culturing the thus transformed strain which is capable of producing the L-amino acid, said two enzymes being highly rate determining enzymes for the biosynthesis of L-amino acid.