Abstract: The electrochemical biosensor strip includes an electrode layer, a spacer, a reagent layer and a cover layer. The electrode layer is disposed on a substrate, and the electrode layer includes a first electrode, a second electrode and a third electrode. The spacer is disposed on the electrode layer, and the spacer includes a first opening, a second opening and a groove which are not communicated with each other. The groove is recessed inwardly from a side of the spacer. The first and second openings are adjacent to the groove. The first opening, the second opening and the groove respectively correspond to and expose the first electrode, the second electrode and the third electrode. The reagent layer is disposed on the first electrode and the second electrode, and the reagent layer is not disposed on the third electrode exposed from the groove. The cover layer is disposed on the spacer.

Abstract: A calibration method for blood glucose of blood sample comprises the following steps: applying a first voltage to a blood sample to obtain an original level of blood glucose of the blood sample; applying a second voltage to a blood sample to obtain a hematocrit index of the blood sample; and processing the hematocrit index and calibrating the original level of blood glucose of the blood sample. The absolute value of the first voltage is lower than 1 volt and is not equal to 0 volt. The absolute value of the second voltage is higher than or equal to 1 volt. A sensing current corresponding to the original blood level and the hematocrit index corresponding to blood sample are obtained by applying at least two-stage voltages in the specific range to the blood sample, thereby calibrating the original blood glucose according to the hematocrit index.

Abstract: A calibration method for blood glucose of a blood sample includes steps of: applying a first voltage to a blood sample to obtain a first blood glucose level; applying a second voltage to the blood sample to obtain a second blood glucose level; applying a third voltage to the blood sample to obtain a hematocrit index of the blood sample; and processing the hematocrit index and calibrating the second blood glucose level. The third voltage is higher than the first voltage. Through this disclosure, user can obtain a sensing current corresponding to the blood glucose level and a hematocrit index corresponding to blood sample by applying at least three-stage voltages within specific ranges to the blood sample, and further calibrate the blood glucose concentration according to the hematocrit index.

Abstract: A calibration method for blood glucose of blood sample comprises the following steps: applying a first voltage to a blood sample to obtain an original level of blood glucose of the blood sample; applying a second voltage to a blood sample to obtain a hematocrit index of the blood sample; and processing the hematocrit index and calibrating the original level of blood glucose of the blood sample. The absolute value of the first voltage is lower than 1 volt and is not equal to 0 volt. The absolute value of the second voltage is higher than or equal to 1 volt. A sensing current corresponding to the original blood level and the hematocrit index corresponding to blood sample are obtained by applying at least two-stage voltages in the specific range to the blood sample, thereby calibrating the original blood glucose according to the hematocrit index.