Abstract: A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.

Abstract: Example methods are provided to improve placement of an adaptor (210,220) to a mobile computing device (100) to measure a test strip (221) coupled to the adaptor (220) with a camera (104) and a screen (108) on a face of the mobile computing device (100). The method may include displaying a light area on a first portion of the screen (108). The first portion may be adjacent to the camera (104). The light area and the camera (104) may be aligned with a key area of the test strip (221) so that the camera (104) is configured to capture an image of the key area. The method may further include providing first guiding information for a user to place the adaptor (210,220) to the mobile computing device (100) according to a position of the light area on the screen (108).

Abstract: A fire extinguisher includes a barrel having a chamber with an opening covered by an upper cover that includes a guide-out passage having a tubular portion with a guide-in groove and an inlet. A guide-out groove is defined above a sealing edge and extends to the outside. A gas inlet unit is mounted to the upper cover and is connected to a high pressure steel bottle. A high pressure gas in the high pressure steel bottle can flow through a gas inlet passage into the chamber. A guide-out unit includes a guiding tube unit connected to the tubular portion and a valve connected to the guiding tube unit. The valve is opened when subjected to a pressure from the high pressure steel bottle. A piston valve normally and sealingly abutting a sealing edge of the tubular portion can be moved to a position in sealing contact with the sealing edge.

Abstract: A fire extinguisher includes a barrel having a chamber with an opening covered by an upper cover that includes a guide-out passage having a tubular portion with a guide-in groove and an inlet. A guide-out groove is defined above a sealing edge and extends to the outside. A gas inlet unit is mounted to the upper cover and is connected to a high pressure steel bottle. A high pressure gas in the high pressure steel bottle can flow through a gas inlet passage into the chamber. A guide-out unit includes a guiding tube unit connected to the tubular portion and a valve connected to the guiding tube unit. The valve is opened when subjected to a pressure from the high pressure steel bottle. A piston valve normally and sealingly abutting a sealing edge of the tubular portion can be moved to a position in sealing contact with the sealing edge.

Abstract: A fire extinguisher includes a body including a chamber receiving fire extinguishing powders. An upper cover includes a gas inlet passage and a gas intake in communication with the gas inlet passage. The upper cover further includes a through-hole aligned with the gas intake. A high-pressure steel bottle is coupled to the upper cover. The spring, when not compressed, holds the piercing needle in the gas intake and the through-hole such that the piercing needle will not pierce into a preserved opening of the high-pressure steel bottle. A pressing rod is pivotably connected to the upper cover. A switch handle is operably connected to the pressing rod. If a pin restraining movement of the switch handle is removed, when switch handle is operated to drive the pressing rod to pivot, the piercing needle is actuated by the pressing rod to pierce into the preserved opening of the high-pressure steel bottle.