Abstract: Manufacturing of a shoe or a portion of a shoe is enhanced by executing various shoe-manufacturing processes in an automated fashion. For example, information describing a shoe part may be determined, such as an identification, an orientation, a color, a surface topography, an alignment, a size, etc. Based on the information describing the shoe part, automated shoe-manufacturing apparatuses may be instructed to apply various shoe-manufacturing processes to the shoe part, such as a pickup and placement of the shoe part with a pickup tool.

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.