Patents by Inventor Chiharu Ohue
Chiharu Ohue has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220349889Abstract: Disclosed is a novel method showing a higher detection sensitivity for hepatitis B virus genotype D than those of known methods. A method of immunoassay of hepatitis B virus core-related antigen uses, as an antibody to be used for the immunoassay, a monoclonal antibody that specifically binds to at least one kind of core-related antigen of hepatitis B virus genotype D, or an antigen-binding fragment thereof, wherein an epitope of the monoclonal antibody or the antigen-binding fragment thereof is a region included in the amino acid sequence from position 31 to 48 of SEQ ID NO:3.Type: ApplicationFiled: September 25, 2020Publication date: November 3, 2022Applicant: FUJIREBIO INC.Inventors: Chiharu OHUE, Shintaro YAGI, Katsumi AOYAGI
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Patent number: 11454632Abstract: Disclosed is a highly sensitive assay method and assay kit for HBsAg, which do not require treatment with a strong acid or alkali in the sample pretreatment, and which is less susceptible to influences by the autoantibodies. The assay method for hepatitis B virus s antigen in a sample separated from a living body includes: a pretreatment step of mixing a sample with a pretreatment reagent containing a reducing agent, to reduce hepatitis B virus s antigen; and an immunoassay step of subjecting the pretreated sample to an immunoassay of hepatitis B virus s antigen using at least one antibody or antigen-binding fragment thereof capable of antigen-antibody reaction with a reduced peptide composed of the amino acids at positions 98 to 179 of hepatitis B virus s antigen.Type: GrantFiled: November 22, 2018Date of Patent: September 27, 2022Assignee: FUJIREBIO INC.Inventors: Chiharu Ohue, Kumiko Iida, Katsumi Aoyagi, Shintaro Yagi
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Publication number: 20220196637Abstract: Disclosed are a measurement method and a measurement reagent for thyroglobulin, which measurement method and measurement reagent enable more accurate measurement of the thyroglobulin level by a single test without being influenced by interference of anti-thyroglobulin antibody, and which measurement method and measurement reagent allow use of even an antibody less resistant to an acidifier and/or a surfactant in an immunoassay. A method of measuring, by an immunoassay, thyroglobulin in a sample separated from a living body includes a pretreatment step of mixing the sample separated from the living body with a pretreatment liquid containing an alkaline substance.Type: ApplicationFiled: May 21, 2020Publication date: June 23, 2022Applicant: FUJIREBIO INC.Inventors: Chiharu OHUE, Shintaro YAGI, Katsumi AOYAGI
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Publication number: 20200371104Abstract: Disclosed is a highly sensitive assay method and assay kit for HBsAg, which do not require treatment with a strong acid or alkali in the sample pretreatment, and which is less susceptible to influences by the autoantibodies. The assay method for hepatitis B virus s antigen in a sample separated from a living body includes: a pretreatment step of mixing a sample with a pretreatment reagent containing a reducing agent, to reduce hepatitis B virus s antigen; and an immunoassay step of subjecting the pretreated sample to an immunoassay of hepatitis B virus s antigen using at least one antibody or antigen-binding fragment thereof capable of antigen-antibody reaction with a reduced peptide composed of the amino acids at positions 98 to 179 of hepatitis B virus s antigen.Type: ApplicationFiled: November 22, 2018Publication date: November 26, 2020Applicant: FUJIREBIO INC.Inventors: Chiharu OHUE, Kumiko IIDA, Katsumi AOYAGI, Shintaro YAGI
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Patent number: 8679762Abstract: [PROBLEMS] To provide a probe useful in the detection of HBV or HBs antigen by which an escape mutant of hepatitis B virus (HBV) possibly occurring in a specimen can be detected; and a method of using the same. [MEANS FOR SOLVING PROBLEMS] A probe capable of recognizing an epitope located on a peptide comprising the amino acid sequence of SEQ ID NO:1; and a method of detecting hepatitis B virus or hepatitis B virus s antigen by using this probe.Type: GrantFiled: September 21, 2005Date of Patent: March 25, 2014Assignee: Advanced Life Science Institute, Inc.Inventors: Noboru Maki, Yasuyuki Fukuda, Tatsuji Kimura, Yoko Oda, Chiharu Ohue, Osamu Kusano
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Publication number: 20110262892Abstract: A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.Type: ApplicationFiled: January 7, 2009Publication date: October 27, 2011Inventors: Katsumi Aoyagi, Chiharu Ohue, Kumiko Iida, Tatsuji Kimura, Shintaro Yagi
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Publication number: 20100291546Abstract: To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule.Type: ApplicationFiled: June 30, 2010Publication date: November 18, 2010Applicant: ADVANCED LIFE SCIENCE INSTITUTE, INC.Inventors: Chiharu Ohue, Noboru Maki, Tatsuji Kimura
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Patent number: 7776542Abstract: A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.Type: GrantFiled: August 4, 1998Date of Patent: August 17, 2010Assignee: Advanced Life Science InstituteInventors: Katsumi Aoyagi, Chiharu Ohue, Kumiko Iida, Tatsuji Kimura, Shintaro Yagi
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Publication number: 20100173298Abstract: The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA is introduced, and a method for screening a drug using the replicon-replicating cell.Type: ApplicationFiled: April 28, 2008Publication date: July 8, 2010Inventors: Kenichi Mori, Noboru Maki, Hiromi Fukai, Chiharu Ohue
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Publication number: 20090017443Abstract: To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule.Type: ApplicationFiled: May 19, 2005Publication date: January 15, 2009Applicant: ADVANCED LIFE SCIENCE INSTITUTE, INC.Inventors: Chiharu Ohue, Noboru Maki, Tatsuji Kimura
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Publication number: 20080193916Abstract: [PROBLEMS] To provide a probe useful in the detection of HBV or HBs antigen by which an escape mutant of hepatitis B virus (HBV) possibly occurring in a specimen can be detected; and a method of using the same. [MEANS FOR SOLVING PROBLEMS]A probe capable of recognizing an epitope located on a peptide comprising the amino acid sequence of SEQ ID NO:1; and a method of detecting hepatitis B virus or hepatitis B virus s antigen by using this probe.Type: ApplicationFiled: September 21, 2005Publication date: August 14, 2008Applicant: ADVANCED LIFE SCIENCE INSTIUTE INC.Inventors: Noboru Maki, Yasuyuki Fukuda, Tatsuji Kimura, Yoko Oda, Chiharu Ohue, Osamu Kusano
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Patent number: 7316905Abstract: A method for measurement of the hepatitis C virus (HCV) characterized by measuring HCV core antigen and HCV core antibody by their binding with probes in the presence of an anionic surfactant or a non-ionic surfactant, or both.Type: GrantFiled: July 30, 1999Date of Patent: January 8, 2008Assignee: Advanced Life Science Institute, Inc.Inventors: Katsumi Aoyagi, Chiharu Ohue, Kumiko Iida, Shintaro Yagi
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Patent number: 6623921Abstract: A method for measurement of the hepatitis C virus (HCV) characterized by measuring HCV core antigen and HCV core antibody by their binding with probes in the presence of an anionic surfactant or a non-ionic surfactant, or both.Type: GrantFiled: April 26, 2002Date of Patent: September 23, 2003Assignee: Advanced Life Science Institute, Inc.Inventors: Katsumi Aoyagi, Chiharu Ohue, Kumiko Iida, Shintaro Yagi