Abstract: The present application provides a purified human protein, DNA fragment encoding the protein, expression vector for the DNA fragment, various cells transformed with the expression vector, and antibody against the protein. The purified protein in this invention is useful as a medicinal or as an antigen for manufacturing the antibody against the proteins. Further, the protein is useful as a search reagent for elucidating the intracellular protein network or as a protein source for screening such a protein as binding with a small molecule medicinal. The human cDNA of this invention is useful as a probe for gene diagnosis or as a gene source for gene therapy. Further, it can be also used as a gene source for mass production of the protein encoded by the cDNA. The expression vector being capable of translating in vitro or expressing the DNA within the host cell can be used for producing the human protein of this invention in vitro or within various host cells.

Abstract: The invention provides a detection method of protein-protein interaction that detects the interaction of a protein X with a protein Y having a reporter function in a eucaryotic cell, which comprises modifying the protein X and/or the protein Y to be expressed with different localization patterns in the cell, co-expressing the protein X and the protein Y in the eucaryotic cell, and identifying the intracellular localization of the protein Y by its reporter function that serves the index thereof. The invention also provides a method for detecting the inhibitory or promotive action of a compound Z on the interaction of the proteins X and Y in a eucaryotic cell.