Patents by Inventor Chin-Kai Chuang
Chin-Kai Chuang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230292720Abstract: The present invention relates to a breeding method for generating gene-edited non-human animals. In particular, the method of the present invention features simultaneous reprograming and gene-editing carried out in somatic cells and subsequent subcloning and genotyping conducted at the in vitro cell stage to obtain precisely gene-edited induced pluripotency stem cells (iPSCs) which are then used in somatic nuclear transfer (SCNT) to generate a precisely gene-edited non-human animal embryo and a resultant gene-edited non-human animal.Type: ApplicationFiled: March 15, 2023Publication date: September 21, 2023Applicant: Agricultural Technology Research InstituteInventors: Ching-Fu Tu, Chin-Kai Chuang, Shu-Hui Peng, Yu-Hsiu Su
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Patent number: 10717994Abstract: The invention provides a recombinant polypeptide X—Y for enhancing cell transduction efficiency of a target agent, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Also provided is a method for enhancing cell transduction efficiency of a target agent, comprising conjugating/attaching said target agent with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Further provided is a pharmaceutical composition comprising a therapeutic agent, wherein said therapeutic agent is modified by conjugating/attaching with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain.Type: GrantFiled: December 15, 2016Date of Patent: July 21, 2020Assignee: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-Kai Chuang, Yu-Hsiu Su, Tzuyin Lin
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Patent number: 10414798Abstract: A synthetic peptide that targets cancer stem cells is provided. The peptide consists of the amino acid sequence of anyone of SEQ ID NO: 1 to SEQ ID NO: 15. Also provided is a composition comprising said synthetic peptide with a therapeutic agent fused thereto, and a pharmaceutically acceptable carrier or diluent. Further provided is a method of screening a peptide specifically targeting to a cancer stem cell. The method comprises the steps of establishing an oligopeptide library by using a phage expression system, contacting the library with a culture of bulk tumor cells of a cancer cell line, contacting the phages which do not bind to the bulk tumor cells with a culture of cancer stem cells of said cancer cell line, and screening a peptide specifically targeting to a cancer stem cell from the phages which bind to the cancer stem cells.Type: GrantFiled: November 22, 2017Date of Patent: September 17, 2019Assignee: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-Kai Chuang, Yu-Hsyu Su, Tai-Yun Lin
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Publication number: 20180170981Abstract: The invention provides a recombinant polypeptide X—Y for enhancing cell transduction efficiency of a target agent, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Also provided is a method for enhancing cell transduction efficiency of a target agent, comprising conjugating/attaching said target agent with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Further provided is a pharmaceutical composition comprising a therapeutic agent, wherein said therapeutic agent is modified by conjugating/attaching with a recombinant polypeptide X—Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain.Type: ApplicationFiled: December 15, 2016Publication date: June 21, 2018Inventors: CHIN-KAI CHUANG, YU-HSIU SU, TZUYIN LIN
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Publication number: 20180072773Abstract: A synthetic peptide that targets cancer stem cells is provided. The peptide consists of the amino acid sequence of anyone of SEQ ID NO: 1 to SEQ ID NO: 15. Also provided is a composition comprising said synthetic peptide with a therapeutic agent fused thereto, and a pharmaceutically acceptable carrier or diluent. Further provided is a method of screening a peptide specifically targeting to a cancer stem cell. The method comprises the steps of establishing an oligopeptide library by using a phage expression system, contacting the library with a culture of bulk tumor cells of a cancer cell line, contacting the phages which do not bind to the bulk tumor cells with a culture of cancer stem cells of said cancer cell line, and screening a peptide specifically targeting to a cancer stem cell from the phages which bind to the cancer stem cells.Type: ApplicationFiled: November 22, 2017Publication date: March 15, 2018Applicant: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-Kai CHUANG, Yu-Hsyu SU, Tai-Yun LIN
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Patent number: 9856291Abstract: A synthetic peptide that targets cancer stem cells is provided. The peptide consists of the amino acid sequence of anyone of SEQ ID NO: 1 to SEQ ID NO: 15. Also provided is a composition comprising said synthetic peptide with a therapeutic agent fused thereto, and a pharmaceutically acceptable carrier or diluent. Further provided is a method of screening a peptide specifically targeting to a cancer stem cell. The method comprises the steps of establishing an oligopeptide library by using a phage expression system, contacting the library with a culture of bulk tumor cells of a cancer cell line, contacting the phages which do not bind to the bulk tumor cells with a culture of cancer stem cells of said cancer cell line, and screening a peptide specifically targeting to a cancer stem cell from the phages which bind to the cancer stem cells.Type: GrantFiled: December 8, 2016Date of Patent: January 2, 2018Assignee: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-Kai Chuang, Yu-Hsyu Su, Tai-Yun Lin
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Publication number: 20170158737Abstract: A synthetic peptide that targets cancer stem cells is provided. The peptide consists of the amino acid sequence of anyone of SEQ ID NO: 1 to SEQ ID NO: 15. Also provided is a composition comprising said synthetic peptide with a therapeutic agent fused thereto, and a pharmaceutically acceptable carrier or diluent. Further provided is a method of screening a peptide specifically targeting to a cancer stem cell. The method comprises the steps of establishing an oligopeptide library by using a phage expression system, contacting the library with a culture of bulk tumor cells of a cancer cell line, contacting the phages which do not bind to the bulk tumor cells with a culture of cancer stem cells of said cancer cell line, and screening a peptide specifically targeting to a cancer stem cell from the phages which bind to the cancer stem cells.Type: ApplicationFiled: December 8, 2016Publication date: June 8, 2017Applicant: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTEInventors: Chin-Kai CHUANG, Yu-Hsyu SU, Tai-Yun LIN
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Publication number: 20150004192Abstract: The invention provides a recombinant polypeptide X-Y for enhancing cell transduction efficiency of a target agent, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Also provided is a method for enhancing cell transduction efficiency of a target agent, comprising conjugating/attaching said target agent with a recombinant polypeptide X-Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain. Further provided is a pharmaceutical composition comprising a therapeutic agent, wherein said therapeutic agent is modified by conjugating/attaching with a recombinant polypeptide X-Y, wherein X is a cell penetrating peptide DPV3, and Y is an Hsp40-J domain.Type: ApplicationFiled: June 30, 2014Publication date: January 1, 2015Inventors: CHIN-KAI CHUANG, YU-HSYU SU, TZUYIN LIN
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Patent number: 7806079Abstract: The present invention discloses a poultry selection method to improve the egg production rate, the method comprises steps of collecting protein samples and egg productivity information of multiple poultry groups, transforming these information into multiple scores, ranks, and their corresponding codes; the next step is to combine the corresponding codes to form regional codes, and compare the matching rate; and select the poultry with the required egg production.Type: GrantFiled: June 19, 2008Date of Patent: October 5, 2010Assignees: National Tsing Hua University, Animal Technology Institute TaiwanInventors: Ming-Li Liou, Chuan-Yi Tang, Yen-Jen Lin, Jyh-Hung Lin, Chin-Kai Chuang, Wen-Chuan Lee
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Patent number: 7759461Abstract: Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein comprising: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein, containing a protein transduction domain (PTD), or a fragment thereof, having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment, containing a J-domain, or a fragment thereof, having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment, containing a target protein or polypeptide.Type: GrantFiled: March 18, 2009Date of Patent: July 20, 2010Assignees: Stresspro Biomedicine Incorporation, Animal Technology Institute TaiwanInventors: Ming-Yu Chen, Chin-Kai Chuang, Yu-Hsyu Su, Chiu-Ting Fan, Jung-Chuan Yu, Wen-Chuan Lee
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Publication number: 20090187004Abstract: Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein comprising: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein, containing a protein transduction domain (PTD), or a fragment thereof, having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment, containing a J-domain, or a fragment thereof, having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment, containing a target protein or polypeptide.Type: ApplicationFiled: March 18, 2009Publication date: July 23, 2009Applicants: STRESSPRO BIOMEDICINE INCORPORATION, ANIMAL TECHNOLOGY INSTITUTE TAIWANInventors: MING-YU CHEN, Chin-Kai Chuang, Yu-Hsyu Su, Chiu-Ting Fan, Jung-Chuan Yu, Wen-Chuan Lee
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Publication number: 20090151640Abstract: The present invention discloses a poultry selection method to improve the egg production rate, the method comprises steps of collecting protein samples and egg productivity information of multiple poultry groups, transforming these information into multiple scores, ranks, and their corresponding codes; the next step is to combine the corresponding codes to form regional codes, and compare the matching rate; and select the poultry with the required egg production.Type: ApplicationFiled: June 19, 2008Publication date: June 18, 2009Inventors: Ming-Li Liou, Chuan-Yi Tang, Yen-Jen Lin, Jyh-Hung Lin, Chin-Kai Chuang, Wen-Chuan Lee
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Patent number: 7524648Abstract: Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.Type: GrantFiled: May 5, 2008Date of Patent: April 28, 2009Assignees: Stresspro Biomedicine Incorporation, Animal Technology Institute TaiwanInventors: Ming-Yu Chen, Chin-Kai Chuang, Yu-Hsyu Su, Chiu-Ting Fan, Jung-Chuan Yu, Wen-Chuan Lee
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Publication number: 20080274508Abstract: Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.Type: ApplicationFiled: May 5, 2008Publication date: November 6, 2008Applicants: STRESSPRO BIOMEDICINE INCORPORATION, ANIMAL TECHNOLOGY INSTITUTE TAIWANInventors: Ming-Yu Chen, Chin-Kai Chuang, Yu-Hsyu Su, Chiu-Ting Fan, Jung-Chuan Yu, Wen-Chuan Lee