Patents by Inventor Chin Toh
Chin Toh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11839874Abstract: A planar modular microfluidic module, system and method for manufacturing such module is provided. The module includes a base layer, and a fluidic layer configured to be attached to and on top of the base layer, whereby the base layer and said fluidic layer configured to create a single basic module, a jacket configured to cover the base module around its lateral sides, in order to provide sturdiness to the basic module and create a jacket covered module. The jacket includes a physical connector configured to enable lateral connection between adjacent modules, the physical connector positioned on at least one lateral side of the jacket covered module, and a fluidic connection port located within said physical connector and configured to enable fluid flow connection between adjacent modules.Type: GrantFiled: October 23, 2018Date of Patent: December 12, 2023Assignee: NATIONAL UNIVERSITY OF SINGAPOREInventors: Yi-Chin Toh, Jun Ye Louis Ong, Christopher Tostado
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Publication number: 20230221299Abstract: There is provided a microfluidic device comprising a first region configured to hold target cells, e.g., tumor cells, a second region configured to hold effector cells, e.g., immune cells, and an array of microstructures disposed between the first and second regions, wherein the first region is in fluid communication with the second region, and wherein the array of microstructures is configured to selectively allow movement of immune cells, from the second region to an interaction zone that is at least partially disposed within the first region, for interaction with tumor cells in the interaction zone. The array of microstructures can be an array of micropillars. Also provided is a chip comprising a plurality of the device and a method of studying interactions of a first cell type with a second cell type.Type: ApplicationFiled: April 22, 2021Publication date: July 13, 2023Inventors: Christopher Peter Tostado, Yi-Chin Toh, Ramanuj Dasgupta
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Publication number: 20200246796Abstract: A planar modular microfluidic module, system and method for manufacturing such module is provided. The module includes a base layer, and a fluidic layer configured to be attached to and on top of the base layer, whereby the base layer and said fluidic layer configured to create a single basic module, a jacket configured to cover the base module around its lateral sides, in order to provide sturdiness to the basic module and create a jacket covered module. The jacket includes a physical connector configured to enable lateral connection between adjacent modules, the physical connector positioned on at least one lateral side of the jacket covered module, and a fluidic connection port located within said physical connector and configured to enable fluid flow connection between adjacent modules.Type: ApplicationFiled: October 23, 2018Publication date: August 6, 2020Inventors: Yi-Chin TOH, Jun Ye Louis ONG, Christopher TOSTADO
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Patent number: 10323228Abstract: Disclosed are methods of differentiating stem cells in order to obtain hepatocyte-like cells, the method comprising the steps of a) subjecting definitive endoderm to at least one epigenetic modulator to obtain hepatoblasts and b) subjecting the hepatoblasts to at least one stem cell differentiation pathway inhibitor to obtain hepatocyte-like cells; wherein steps a) and b) do not comprise the use of a growth factor. In one preferred embodiment, the epigenetic modulator may be sodium butyrate and/or DMSO and the stem cell differentiation pathway inhibitor may be SB431542 and/or DMSO. Also disclosed are hepatocyte-like cells obtained from the method and uses of these cells such as drug screening.Type: GrantFiled: September 17, 2015Date of Patent: June 18, 2019Assignee: Agency for Science, Technology and ResearchInventors: Hanry Yu, Derek Phan, Yi-chin Toh, Farah Tasnim
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Patent number: 9958433Abstract: A method and system of in vitro developmental toxicity testing comprising the steps of micropatterning an extracellular matrix; growing embryonic stem cells on the micropatterned extracellular matrix in the presence of mesoendodermal induction and testing for change of the geometrical mesoendoderm structure in the presence or absence of a test compound wherein (1) a decrease in mesoendodermal differentiation and/or (2) a change in morphology of the geometrical mesoendoderm structure in the presence of the test compound compared to cells in the absence of the test compound indicates that the test compound is a developmental toxic agent.Type: GrantFiled: September 30, 2013Date of Patent: May 1, 2018Assignee: Agency for Science, Technology and ResearchInventors: Hanry Yu, Yi-Chin Toh, Jiangwa Xing
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Publication number: 20170260503Abstract: Disclosed are methods of differentiating stem cells in order to obtain hepatocyte-like cells, the method comprising the steps of a) subjecting definitive endoderm to at least one epigenetic modulator to obtain hepatoblasts and b) subjecting the hepatoblasts to at least one stem cell differentiation pathway inhibitor to obtain hepatocyte-like cells; wherein steps a) and b) do not comprise the use of a growth factor. In one preferred embodiment, the epigenetic modulator may be sodium butyrate and/or DMSO and the stem cell differentiation pathway inhibitor may be SB431542 and/or DMSO. Also disclosed are hepatocyte-like cells obtained from the method and uses of these cells such as drug screening.Type: ApplicationFiled: September 17, 2015Publication date: September 14, 2017Inventors: Hanry YU, Derek PHAN, Yi-chin TOH, Farah TASNIM
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Patent number: 9663874Abstract: There is provided a device, and related method and uses, for drawing a polymer fiber, the device comprising: a. at least two polymer compartments, wherein each polymer compartment is capable of retaining a polymer solution, and wherein adjacent compartments comprise different polymer solutions; and b. a slider comprising at least one prong, wherein the prong is capable of contacting the different polymer solutions, and wherein the slider is arranged in a retractable manner from the at least two polymer compartments. There is further provided a system and a related method for manufacturing a polymer fiber.Type: GrantFiled: March 18, 2013Date of Patent: May 30, 2017Assignee: Agency for Science, Technology and ResearchInventors: Andrew Chwee Aun Wan, Meng Fatt Leong, Tze Chiun Lim, Jackie Y. Ying, Jerry Kah Chin Toh
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Publication number: 20150284669Abstract: A method of culturing cells or tissues is provided. The method comprises a) providing a support, wherein the support is an optical medium with a patterned surface; b) contacting the support with cells and a culture medium; and c) culturing the cells under suitable cultivation conditions. A device for cell or tissue culture comprising an optical medium, wherein a patterned surface of the optical medium forms a support upon which cells are cultivated, is also provided.Type: ApplicationFiled: November 8, 2012Publication date: October 8, 2015Inventors: Deepak Choudhury, Chukwuemeka Anene-Nzelu, Hanry Yu, Yi-Chin Toh, Hwa Liang Leo, Sum Huan Ng
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Publication number: 20150276711Abstract: A method and system of in vitro developmental toxicity testing comprising the steps of micropatterning an extracellular matrix; growing embryonic stem cells on the micropatterned extracellular matrix in the presence of mesoendermal induction and testing for change of the geometrical mesoendoderm structure in the presence or absence of a test compound wherein (1) a decrease in mesoendodermal differentiation and/or (2) a change in morphology of the geometrical mesoendoderm structure in the presence of the test compound compared to cells in the absence of the test compound indicates that the test compound is a developmental toxic agent.Type: ApplicationFiled: September 30, 2013Publication date: October 1, 2015Applicant: Agency for Science, Technology and ResearchInventors: Hanry Yu, Yi-Chin Toh, Jiangwa Xing
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Patent number: 9029099Abstract: A fibrous structure comprising an assembly of hair follicle cells within a fibrous matrix.Type: GrantFiled: April 22, 2013Date of Patent: May 12, 2015Inventors: Andrew Chwee Aun Wan, Tze Chiun Lim, Meng Fatt Leong, Jackie Y. Ying, Jerry Kah Chin Toh
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Publication number: 20150050711Abstract: There is provided a device, and related method and uses, for drawing a polymer fiber, the device comprising: a. at least two polymer compartments, wherein each polymer compartment is capable of retaining a polymer solution, and wherein adjacent compartments comprise different polymer solutions; and b. a slider comprising at least one prong, wherein the prong is capable of contacting the different polymer solutions, and wherein the slider is arranged in a retractable manner from the at least two polymer compartments. There is further provided a system and a related method for manufacturing a polymer fiber.Type: ApplicationFiled: March 18, 2013Publication date: February 19, 2015Inventors: Andrew Chwee Aun Wan, Meng Fatt Leong, Tze Chiun Lim, Jackie Y. Ying, Jerry Kah Chin Toh
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Publication number: 20140011227Abstract: A fibrous structure comprising an assembly of hair follicle cells within a fibrous matrix.Type: ApplicationFiled: April 22, 2013Publication date: January 9, 2014Applicant: Agency for Science, Technology and ResearchInventors: Andrew Chwee Aun WAN, Tze Chiun LIM, Meng Fatt LEONG, Jackie Y. YING, Jerry Kah Chin TOH
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Publication number: 20130288287Abstract: A fibrous structure comprising an assembly of hair follicle cells within a fibrous matrix.Type: ApplicationFiled: March 15, 2013Publication date: October 31, 2013Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCHInventors: Andrew Chwee Aun WAN, Tze Chiun Lim, Meng Fatt Leong, Jackie Y. Ying, Jerry Kah Chin TOH
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Publication number: 20130202672Abstract: The present invention relates to a fiber-assembled tissue construct comprising at least one sinusoid unit, the unit comprising at least two polymeric fibers arranged in a sinusoid structure and fused together, each of said fibers comprising a porous matrix supporting biological components encapsulated in the fiber, wherein the biological components are patterned in three-dimensions within the construct.Type: ApplicationFiled: February 19, 2010Publication date: August 8, 2013Inventors: Andrew Chwee Aun Wan, Meng Fatt Leong, Jackie Y. Ying, Jerry Kah Chin Toh
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Patent number: 8343742Abstract: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold.Type: GrantFiled: January 25, 2010Date of Patent: January 1, 2013Assignee: Agency for Science, Technology and ResearchInventors: Hanry Yu, Yi Chin Toh
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Publication number: 20100136649Abstract: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold.Type: ApplicationFiled: January 25, 2010Publication date: June 3, 2010Applicant: Agency for Science, Technology and ResearchInventors: Hanry Yu, Yi Chin Toh
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Patent number: 7704714Abstract: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold.Type: GrantFiled: July 26, 2004Date of Patent: April 27, 2010Assignee: Agency for Science, Technology & ResearchInventors: Hanry Yu, Yi Chin Toh
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Publication number: 20080233607Abstract: The invention provides cell culture devices comprising a channel, the channel comprising one or more inlets and one or more outlets, and a cell retention chamber defined by an internal surface of the channel and a plurality of projections extending therefrom. The invention further provides methods of use relating to such cell culture devices.Type: ApplicationFiled: November 10, 2005Publication date: September 25, 2008Inventors: Hanry Yu, Yi-Chin Toh, San San Susanne Ng
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Publication number: 20070210428Abstract: Embodiments of the present invention provide a die stack including a first substrate, a first die bonded to the first substrate, a second substrate having a cavity sized and shaped to fit over the first die, and a second die bonded to at least a portion of a rim of the cavity.Type: ApplicationFiled: March 9, 2006Publication date: September 13, 2007Inventors: Wooi Tan, Chee Chen, Chin Toh, Chiang Lim