Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.

Abstract: A magnetism characteristic detection method, a magnetism characteristic detection apparatus, an imaging apparatus and an imaging method are provided, where magnetism detection and imaging are based on an integrated excitation field of a direct current (DC) magnetic field and an oscillation wave. The magnetism detection method includes the following steps. A DC magnetic field is selectively applied to an object. Further, an oscillation wave is provided to the object, where the oscillation wave is a sound wave or an ultrasound wave. Then, a magnetism characteristic variation of the object is detected.

Abstract: The present invention proposes a method of using oligonucleotide aptamers binding with certain specific biomolecules associated with a disease to purify the specific biomolecules from disease patient's samples and to identify biomarkers for the particular disease. The present invention describes a method using the magnetic assisted rapid aptamer selection (MARAS) to select aptamers having high binding affinity for certain disease-related biomolecules and not for other non-disease-related biomolecules from nucleic acid libraries. Meanwhile, the present invention also proposes a method of using the aptamer obtained above as a capture ligand to purify certain specific biomolecules related to a disease from disease patients' samples. Further, the present invention describes the use of the obtained aptamer as the capture ligand to purify biomolecules with a specific binding affinity range by applying an oscillating magnetic field range as a virtual filter.

Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.

Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.

Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.

Abstract: A magnetism characteristic detection method, a magnetism characteristic detection apparatus, an imaging apparatus and an imaging method are provided, where magnetism detection and imaging are based on an integrated excitation field of a direct current (DC) magnetic field and an oscillation wave. The magnetism detection method includes the following steps. A DC magnetic field is selectively applied to an object. Further, an oscillation wave is provided to the object, where the oscillation wave is a sound wave or an ultrasound wave. Then, a magnetism characteristic variation of the object is detected.

Abstract: A magnetic-assisted rapid aptamer selection (MARAS) protocol for screening DNA aptamer is proposed. The MARAS protocol is able to efficiently generate aptamers with high affinity and specificity. A rotating magnetic field or alternating magnetic field was used in combination with target-bound magnetic micro-particles or nanoparticles to select DNA aptamers having desirable affinity and specificity to the target.

Abstract: Disclosed is an apparatus for measuring ac magnetization at mixture frequency. The apparatus includes an ac generating unit for generating at least a first current with a frequency f1 and a second current with a frequency f2. The apparatus further includes a co-axial solenoid unit, driven by the first and second ac currents, to generate a first magnetic field and a second magnetic field. A pick-up solenoid is for disposing sample for detecting an ac magnetization of the sample and multiple frequency-component signals corresponding to various frequency combinations of f1 and f2 are output. The apparatus further includes a signal processing circuit for receiving the frequency-component signals, where the signal processing circuit obtains the ac magnetization of the sample at a target frequency of (?Tf1+?Tf2), which ?T and ?T are positive integers and the frequency f1 and the frequency f2 are two different frequencies.

Abstract: A method for suppressing non-specific bindings between molecules includes providing magnetic particles dispersed in a liquid, wherein each of the magnetic particles has a magnetization. The magnetic particles are coated with coating molecules. Binding molecules mixed of first-type binding molecules and second-type binding molecules are applied to the liquid, wherein the coating molecules are specifically binding with the first-type binding molecules and non-specifically binding with the second-type binding molecules. An alternating current (ac) magnetic field is applied at an axis with a frequency level, wherein the frequency level causes suppression of the second-type binding molecules in binding with the coating molecules.

Abstract: The present invention provides a probe-type SQUID system to detect magnetic particles stored in the living organisms or magnetic-labeling indicators for immunoassays and tumor or other applications. The probe-type SQUID system comprises a probe union, a SQUID union and a connecting electrically conducting wires such as copper wires, wherein the probe union is coupled with a cooling module such as TE cooler module to avoid power heating so that the probe can approach to the living organism to detect magnetic particles with high-sensitivity.

Abstract: A method for suppressing non-specific bindings between molecules includes providing magnetic particles dispersed in a liquid, wherein each of the magnetic particles has a magnetization. The magnetic particles are coated with coating molecules. Binding molecules mixed of first-type binding molecules and second-type binding molecules are applied to the liquid, wherein the coating molecules are specifically binding with the first-type binding molecules and non-specifically binding with the second-type binding molecules. An alternating current (ac) magnetic field is applied at an axis with a frequency level, wherein the frequency level causes suppression of the second-type binding molecules in binding with the coating molecules.

Abstract: A method to quantitatively measure an amount of bio-molecules in a sample includes providing a solution having magnetic nanoparticles; coating bioprobe molecules to surfaces of the magnetic nanoparticles in the solution; measuring a first alternating current (ac) magnetization of the solution at a mixture frequency (?f1+?f2), wherein ? or ? is independently an integer larger than zero; adding a sample containing the bio-molecules to be detected to the solution, so that the biomolecules in the sample conjugate with the bioprobe molecules coated on the nanoparticles; and measuring a second ac magnetization of the solution at the mixture frequency (?f1+?f2) after adding the sample and incubation, so as to obtain an ac magnetization reduction at the mixture frequency (?f1+?f2) between the first and the second magnetization to determine the amount of the bio-molecules.

Abstract: A method for measuring a concentration of nucleic acids is described. The method includes providing bioprobe molecules that include single-stranded nucleic acids, wherein the bioprobe molecules are conjugated with magnetic beads in a solution. A sample of single-stranded target nucleic acids is added to solution, where the single-stranded target nucleic acids hybridize with the single-stranded nucleic acids of the bioprobe molecules. A reduction of the ac (alternating current) magnetic susceptibility of the solution prior and after the addition of the sample to the solution is determined.