Abstract: The present invention provides a vortexed-acoustic-field method for creating cell permeability and opening blood-brain barrier, which comprises using an ultrasonic transducer; using a probe emitting a vortexed acoustic field, the probe emitting a vortexed acoustic field having a piezoelectric patch comprising at least one inner channel, where when there is a plurality of inner channels, a phase difference is generated between each two channels, which is used by the ultrasonic transducer to generate a vortex in an acoustic channel; and generating a vortexed acoustic field to enable the cells to have permeability, where the vortexed acoustic field has the following parameters: frequency: 20 kHz to 20 MHz, and acoustic pressure: 0.1 to 10 MPa.