Abstract: An emotion regulation system and a regulation method thereof are disclosed. A physiological emotion processing device of the emotion regulation system comprises an emotion feature processing unit and a physiological emotion analyzing unit. The emotion feature processing unit outputs a physiological feature signal according to a physiological signal generated by a user listening to a first music signal. The physiological emotion analyzing unit analyzes the user's physiological emotion according to the physiological feature signal and generates a physiological emotion state signal. A music feature processing unit of a musical emotion processing device obtains corresponding music feature signals from music signals. A music emotion analyzing processing unit analyzes the music feature signals to obtain musical emotions of the music signals and outputs a corresponding second music signal to the user according to the physiological emotion state signal and a target emotion.