Abstract: Systems and methods for near-infrared fluorescence (NIRF) imaging and frequency-domain photon migration (FDPM) measurements. An optical tomography system includes a bed, a wheel, a light source, an image detector, and radio frequency (RF) circuitry. The bed is configured to support an object to be imaged. The wheel is configured to rotate about the bed. The light source is coupled to the wheel. The image detector is coupled to the wheel and disposed to capture images of the object. The RF circuitry is coupled to the light source and the image detector. The RF circuitry is configured to simultaneously generate a modulation signal to modulate the light source, and generate a demodulation signal to modulate a gain of the image detector.