Abstract: A method for forming one or more nano-sized patterns using one or more molecule species overlying a substrate structure. In a preferred embodiment, the pattern or patterns relate to an array of biological molecules (e.g., DNA, small molecule(s), protein(s), ligand(s)). The method applies a probe tip (e.g., atomic force microscope probe (AFM probe)) within a vicinity of a first spatial region of a surface region of a substrate member, which is characterized by a first characteristic, e.g., hydrophobic, hydrophilic, partially hydrophobic, partially hydrophilic. In a specific embodiment, the probe tip is in a direction (e.g., normal, at an angle toward) toward the spatial region on the surface region. The method includes transferring one of more of a plurality of molecules characterized by a second characteristic through a fluid medium comprising one or more surfactant species (e.g.