Abstract: A method for detecting a target nucleic acid fragment includes the following steps: preparing a nucleic acid probe and an intercalating dye configured to bind to double-stranded nucleic acids, wherein the nucleic acid probe includes a nucleic acid chain, a fluorescent reporter, and a fluorescent quencher, the fluorescent reporter is conjugated to a first end of the nucleic acid chain, and the fluorescent quencher is conjugated to a second end of the nucleic acid chain opposing to the first end; binding the nucleic acid probe to the target nucleic acid fragment to form a partial double stranded structure; intercalating the intercalating dye in the partial double stranded structure, so that, after the intercalating dye is excited by an irradiation, the fluorescent reporter is excited through the intercalating dye to emit a fluorescence signal; and detecting the target nucleic acid fragment according to the fluorescence signal.

Abstract: Fetuin-beads, a manufacturing method thereof and a method of concentrating and detecting influenza virus by the fetuin-beads are disclosed. The method of concentrating and detecting an influenza virus by the fetuin-beads comprises the steps of preparing fetuin-beads; mixing the fetuin-beads and a solution capable of providing salt ions to obtain a fetuin-bead solution; mixing the fetuin-bead solution and a sample comprising the influenza virus to concentrate the influenza virus onto the fetuin-beads to obtain a plurality of fetuin bead-influenza virus combinations; and collecting the fetuin bead-influenza virus combinations and using a virus testing method to detect the influenza virus on the fetuin bead-influenza virus combinations. Therefore, an easy and fast method to detect the influenza virus with timeliness and high accuracy is provided.

Abstract: Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid (PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the pair of primers and the PNA.

Abstract: Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid (PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the pair of primers and the PNA.